Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Esophageal squamous cell carcinomas with DNA replication errors (RER+) areassociated with p16/pRb loss and wild-type p53
Autore:
Mathew, R; Arora, S; Mathur, M; Chattopadhyay, TK; Ralhan, R;
Indirizzi:
All India Inst Med Sci, Dept Biochem, New Delhi 110029, India All India Inst Med Sci New Delhi India 110029 m, New Delhi 110029, India All India Inst Med Sci, Dept Pathol, New Delhi 110029, India All India Inst Med Sci New Delhi India 110029 l, New Delhi 110029, India All India Inst Med Sci, Dept Surg Gastroenterol, New Delhi 110029, India All India Inst Med Sci New Delhi India 110029 l, New Delhi 110029, India
Titolo Testata:
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
fascicolo: 10, volume: 127, anno: 2001,
pagine: 603 - 612
SICI:
0171-5216(200110)127:10<603:ESCCWD>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-SUPPRESSOR GENES; MICROSATELLITE INSTABILITY; BREAST CARCINOMAS; COLORECTAL-CANCER; MOLECULAR-BIOLOGY; FREQUENT LOSS; MUTATION; EXPRESSION; HETEROZYGOSITY; PROFILE;
Keywords:
esophageal cancer; microsatellite instability; RER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Ralhan, R All India Inst Med Sci, Dept Biochem, New Delhi 110029, India All India Inst Med Sci New Delhi India 110029 hi 110029, India
Citazione:
R. Mathew et al., "Esophageal squamous cell carcinomas with DNA replication errors (RER+) areassociated with p16/pRb loss and wild-type p53", J CANC RES, 127(10), 2001, pp. 603-612

Abstract

Purpose: Microsatellite instability (MSI) as a determinant of propensity to esophageal squamous cell carcinoma (ESCC at seven microsatellite markers at 2p (2p 15-16), 3p (3p13, 3p 14.1-3, 3p25, and 3p26) and 16q (16q12.1-3) was investigated to analyze their putative role as indicators of predisposition to esophageal malignancies. Methods: Seven microsatellite loci were amplified by polymerase chain reaction, from surgically resected tumor tissues from 30 ESCC patients from Indian population. to assess the loss of heterozygosity (LOH) and replication error repeats (RER) and to correlate these alterations with aberrations in major cell cycle regulatory proteins and histopathological parameters. Results: LOH and RER analyses at these loci demonstrated moderate microsatellite alterations, suggesting the involvement of MSI in esophageal tumorigenesis in a subset of the Indian population. MSIdefined as RER in at least two or more of the loci studied, was observed in ten of 30 (33%) patients. Twenty-two of 30 patients (73%) showed LOH at one or more loci, while 17 of the 30 patients (60%) showed RER in at least one of the loci studied. RER-positive patients showed a trend towards betterprognosis when compared to RER-negative patients. NISI demonstrated a significant association with concomitant loss of p16 and pRb (p16-/pRb- phenotype) (P=0.046). Interestingly, we observed an inverse correlation between MSI and p53 mutations (P=0.03) suggesting that NISI may provide a p53-independent pathway for esophageal tumorigenesis in RER+ patients. MSI showed a trend towards longer survival and absence of distant organ metastasis (P=0.06). Conclusions: The present study demonstrates the probable role of MSI in esophageal squamous cell carcinoma in the Indian population. Instability associated with the repetitive sequences - the revealing marks of loss of DNAreplication fidelity may serve as an indicator of predisposition to esophageal cancer.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 15:42:44