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Titolo:
Effects of ovariectomy and estrogen on ischemia-reperfusion injury in hindlimbs of female rats
Autore:
Stupka, N; Tiidus, PM;
Indirizzi:
Wilfrid Laurier Univ, Dept Kinesiol & Phys Educ, Waterloo, ON N2L 3C5, Canada Wilfrid Laurier Univ Waterloo ON Canada N2L 3C5 erloo, ON N2L 3C5, Canada Univ Waterloo, Dept Kinesiol, Waterloo, ON N2L 3C5, Canada Univ Waterloo Waterloo ON Canada N2L 3C5 ol, Waterloo, ON N2L 3C5, Canada
Titolo Testata:
JOURNAL OF APPLIED PHYSIOLOGY
fascicolo: 4, volume: 91, anno: 2001,
pagine: 1828 - 1835
SICI:
8750-7587(200110)91:4<1828:EOOAEO>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSIENT FOREBRAIN ISCHEMIA; CALPAIN SMALL-SUBUNIT; GROWTH-FACTOR-I; SKELETAL-MUSCLE; MYELOPEROXIDASE ACTIVITY; PROTEASE CALPAIN; OXIDATIVE STRESS; ACETYL PEPTIDES; EXERCISE; DAMAGE;
Keywords:
inflammatory cells; myeloperoxidase; calpain; creatine kinase; skeletal muscle;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Tiidus, PM Wilfrid Laurier Univ, Dept Kinesiol & Phys Educ, Waterloo, ON N2L 3C5, Canada Wilfrid Laurier Univ Waterloo ON Canada N2L 3C5 2L 3C5, Canada
Citazione:
N. Stupka e P.M. Tiidus, "Effects of ovariectomy and estrogen on ischemia-reperfusion injury in hindlimbs of female rats", J APP PHYSL, 91(4), 2001, pp. 1828-1835

Abstract

The effects of estrogen and ovariectomy on indexes of muscle damage after 2 h of complete hindlimb ischemia and 2 h of reperfusion were investigated in female Sprague-Dawley rats. The rats were assigned to one of three experimental groups: ovariectomized with a 17 beta -estradiol pellet implant (OE), ovariectomized with a placebo pellet implant (OP), or control with intact ovaries (R). It was hypothesized that following ischemia-reperfusion (I/R), muscle damage indexes [serum creatine kinase (CK) activity, calpain-likeactivity, inflammatory cell infiltration, and markers of lipid peroxidation (thiobarbituric-reactive substances)] would be lower in the OE and R ratscompared with the OP rats due to the protective effects of estrogen. SerumCK activity following I/R was greater (P < 0.01) in the R rats vs. OP ratsand similar in the OP and OE rats. Calpain-like activity was greatest in the R rats (P < 0.01) and similar in the OP and OE rats. Neutrophil infiltration was assessed using the myeloperoxidase (MPO) assay and immunohistochemical staining for CD43-positive (CD43+) cells. MPO activity was lower (P < 0.05) in the OE rats compared with any other group and similar in the OP and R rats. The number of CD43+ cells was greater (P < 0.01) in the OP rats compared with the OE and R rats and similar in the OE and R rats. The OE rats had lower (P < 0.05) thiobarbituric-reactive substance content following I/R compared with the R and OP rats. Indexes of muscle damage were consistently attenuated in the OE rats but not in the R rats. A 10-fold difference in serum estrogen content may mediate this. Surprisingly, serum CK activityand muscle calpain-like activity were lower (P < 0.05) in the OP rats compared with the R rats. Increases in serum insulin-like growth factor-1 content (P < 0.05) due to ovariectomy were hypothesized to account for this finding. Thus both ovariectomy and estrogen supplementation have differential effects on indexes of I/R muscle damage.

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Documento generato il 29/01/20 alle ore 18:57:28