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Titolo:
Decreased non-MHC-restricted (CD56(+)) killer cell cytotoxicity after spaceflight
Autore:
Mehta, SK; Kaur, I; Grimm, EA; Smid, C; Feeback, DL; Pierson, DL;
Indirizzi:
NASA, Lyndon B Johnson Space Ctr, Life Sci Res Labs, Houston, TX 77058 USANASA Houston TX USA 77058 e Ctr, Life Sci Res Labs, Houston, TX 77058 USA Enterprise Advisory Serv Inc, Houston, TX 77058 USA Enterprise Advisory Serv Inc Houston TX USA 77058 , Houston, TX 77058 USA Univ Texas, MD Anderson Canc Ctr, Dept Tumor Biol, Houston, TX 77030 USA Univ Texas Houston TX USA 77030 r, Dept Tumor Biol, Houston, TX 77030 USA
Titolo Testata:
JOURNAL OF APPLIED PHYSIOLOGY
fascicolo: 4, volume: 91, anno: 2001,
pagine: 1814 - 1818
SICI:
8750-7587(200110)91:4<1814:DN(KCC>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNE CHANGES; DURATION MISSIONS; SPACE-FLIGHT; RADIOIMMUNOASSAY; REACTIVATION; ASTRONAUTS; PLASMA; SERUM;
Keywords:
space immunology; microgravity; non-major histocompatibility complex-restricted killer cell;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Pierson, DL NASA, Lyndon B Johnson Space Ctr, Life Sci Res Labs, Mail CodeSF24, Houston, TX 77058 USA NASA Mail Code SF24 Houston TX USA 77058 Houston, TX 77058 USA
Citazione:
S.K. Mehta et al., "Decreased non-MHC-restricted (CD56(+)) killer cell cytotoxicity after spaceflight", J APP PHYSL, 91(4), 2001, pp. 1814-1818

Abstract

Cytotoxic activity of non-major histocompatibility complex-restricted (CD56(+)) (NMHC) killer cells and cell surface marker expression of peripheral blood mononuclear cells were determined before and after spaceflight. Ten astronauts (9 men, 1 woman) from two space shuttle missions (9- and 10-day duration) participated in the study. Blood samples were collected 10 days before launch, within 3 h after landing, and 3 days after landing. All peripheral blood mononuclear cell preparations were cryopreserved and analyzed simultaneously in a 4-h cytotoxicity Cr-51 release assay using K562 target cells. NMHC killer cell lytic activity was normalized per 1,000 CD56(+) cells. When all 10 subjects were considered as one study group, NMHC killer cellnumbers did not change significantly during the three sampling periods, but at landing lytic activity had decreased by similar to 40% (P < 0.05) frompreflight values. Nine of ten astronauts had decreased lytic activity immediately after flight. NMHC killer cell cytotoxicity of only three astronauts returned toward preflight values by 3 days after landing. Consistent withdecreased NMHC killer cell cytotoxicity, urinary cortisol significantly increased after landing compared with preflight levels. Plasma cortisol and ACTH levels at landing were not significantly different from preflight values. No correlation of changes in NMHC killer cell function or hormone levelswith factors such as age, gender, mission, or spaceflight experience was found. After landing, expression of the major lymphocyte surface markers (CD3, CD4, CD8, CD14, CD16, CD56), as determined by flow cytometric analysis, did not show any consistent changes from measurements made before flight.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 13:20:25