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Titolo:
Differentiation of Th1 and Th2 cells in lymph nodes and spleens of mice during experimental autoimmune uveoretinitis
Autore:
Takeuchi, M; Yokoi, H; Tsukahara, R; Sakai, J; Usui, M;
Indirizzi:
Tokyo Med Univ, Dept Ophthalmol, Shinjuku Ku, Tokyo 1600023, Japan Tokyo Med Univ Tokyo Japan 1600023 ol, Shinjuku Ku, Tokyo 1600023, Japan
Titolo Testata:
JAPANESE JOURNAL OF OPHTHALMOLOGY
fascicolo: 5, volume: 45, anno: 2001,
pagine: 463 - 469
SICI:
0021-5155(200109/10)45:5<463:DOTATC>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENETIC SUSCEPTIBILITY; FUNCTIONAL-PROPERTIES; T-CELLS; SUPPRESSION; EXPRESSION; DISEASE; UVEITIS; IL-10;
Keywords:
autoimmune disease; experimental autoimmune uveoretinitis; immunoregulation; Th1 cells; Th2 cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Takeuchi, M Tokyo Med Univ, Dept Ophthalmol, Shinjuku Ku, 6-7-1 Nishishinjuku, Tokyo 1600023, Japan Tokyo Med Univ 6-7-1 Nishishinjuku Tokyo Japan 1600023 Japan
Citazione:
M. Takeuchi et al., "Differentiation of Th1 and Th2 cells in lymph nodes and spleens of mice during experimental autoimmune uveoretinitis", JPN J OPHTH, 45(5), 2001, pp. 463-469

Abstract

Purpose: Experimental autoimmune uveoretinitis (EAU) is a T-cell-mediated autoimmune disease that can be elicited in susceptible rodent strains by immunization with a retinal autoantigen, such as interphotoreceptor retinoid-binding protein (IRBP). In this study, we investigated whether there is a correlation between inflammation in the eye and T-helper (Th)1- and Th2-typeresponses in the lymph nodes and the spleen after immunization of B10.A mice with IRBP. Methods: B10.A mice were immunized with IRBP emulsified with complete Freund's adjuvant (CFA), and eyes were then enucleated for histological examination of EAU at 1, 2. 4, 6, or 8 weeks after immunization. In addition, lymph node cells and spleen cells were collected, and cultured with IRBP to measure T-cell proliferation responses and Th1-type (interleukin [IL]-2, interferon [IFN]-gamma), Th2-type (IL-4, IL-10) cytokine production. Results: Pathologically, severe ocular inflammation occurred 2 weeks afterIRBP immunization, persisted for 2 weeks, and then gradually resolved. Interleukin-2 and IFN-gamma production were observed in draining lymph node cells at 1 and 2 weeks after IRBP immunization. Those responses then diminished. whereas IFN-gamma production by spleen cells was observed from week 1, peaked at week 4. and gradually decreased. Alternatively, significant production of IL-4 or IL-10 by draining lymph node cells was not detected at anytime point. Both IL-4 and IL-10 production by spleen cells was observed atweek 6. Conclusions: Th1-type responses were observed early in draining lymph nodes, then in the spleen after IRBP immunization. The levels of IFN-gamma production by spleen cells reflected the severity of EAU, confirming their pathogenic role in this disease. Th2-type responses were generated in the spleen only as the disease receded, suggesting a role for Th2 cells in the spontaneous termination of EAU. Jpn J Ophthalmol 2001;45:463-469 (C) 2001 Japanese Ophthalmological Society.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 20:15:47