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Titolo:
Skeletal muscle protein loss due to D-penicillamine results from reduced protein synthesis
Autore:
Preedy, VR; Wassif, WS; Baldwin, D; Jones, J; Falkous, G; Marway, JS; Mantle, D; Scott, DL;
Indirizzi:
Univ London Kings Coll, Dept Nutr & Dietet, Mol & Cellular Prot Biochem Lab, London SE1 9NN, England Univ London Kings Coll London England SE1 9NN b, London SE1 9NN, England Univ London Kings Coll, Sch Med & Dent, Dept Clin Biochem, London SE5 9PJ,England Univ London Kings Coll London England SE5 9PJ em, London SE5 9PJ,England Univ London Kings Coll, Sch Med & Dent, Dept Med, London SE5 9PJ, England Univ London Kings Coll London England SE5 9PJ d, London SE5 9PJ, England Inst Child Hlth, Dept Growth & Dev, London WC1N 1EH, England Inst Child Hlth London England WC1N 1EH & Dev, London WC1N 1EH, England Newcastle Gen Hosp, Reg Neurosci Ctr, Dept Neurochem, Newcastle Upon Tyne NE4 6BE, Tyne & Wear, England Newcastle Gen Hosp Newcastle Upon Tyne Tyne & Wear England NE4 6BE ngland
Titolo Testata:
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
fascicolo: 10, volume: 33, anno: 2001,
pagine: 1013 - 1026
SICI:
1357-2725(200110)33:10<1013:SMPLDT>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBER-TYPE COMPOSITION; FREE AMINO-ACIDS; 9 RAT MUSCLES; WILSONS-DISEASE; RHEUMATOID-ARTHRITIS; TURNOVER; TISSUES; METALLOTHIONEIN; ACETALDEHYDE; FIBROBLASTS;
Keywords:
protein synthesis; skeletal muscle; rat; proteases; carbonyl;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Preedy, VR Univ London Kings Coll, Dept Nutr & Dietet, Mol & Cellular ProtBiochem Lab, 150 Stamford St, London SE1 9NN, England Univ London Kings Coll 150 Stamford St London England SE1 9NN
Citazione:
V.R. Preedy et al., "Skeletal muscle protein loss due to D-penicillamine results from reduced protein synthesis", INT J BIO C, 33(10), 2001, pp. 1013-1026

Abstract

Reports in the literature indicate that (lie trifunctional amino acid D-penicillamine (D-P) induces a variety of muscle abnormalities, although the mechanisms are unknown. We hypothesised that defects may also arise due to the effects of D-P on rates of protein synthesis, possibly via changes in muscle metal composition. Male Wistar rats were injected with D-P at doses of50 and 500 mg/kg body weight, i.p. Rats designated as controls were injected with 0.15 mol/l NaCl. After 24 h, there were reductions in muscle protein contents, protein synthetic capacities (RNA:protein ratio), fractional rates of protein synthesis, synthesis rates per unit RNA and synthesis rates per unit DNA in skeletal muscles of D-P treated rats. There were no statistically significant differences between the responses of the muscles containing a predominance of either Type I (represented by the soleus) or Type II (represented by the plantaris fibres. In general, intracellular amino acidswere not significantly affected by D-P treatment. Changes in muscle metalsincluded significant reductions in copper, iron and manganese, without alterations in zinc or magnesium. In liver D-P reduced copper and iron though zinc, manganese and magnesium were unaffected. These effects of D-P on muscle may have been direct, as plasma indices of liver (activities of alkalinephosphatase and alanine amino transferase) and kidney (urea, creatinine and electrolytes) damage were not significantly altered by D-P treatment. Plasma levels of corticosterone, insulin and free T-3 were also not significantly affected by D-P treatment. Muscle protein carbonyl concentrations, an index of free radical activity, were similarly unaffected. This is the firstreport of reduced rates of muscle protein synthesis in D-P treatment. Our data suggests that the reduced rates of muscle protein synthesis may contribute to, or reflect, the muscle abnormalities observed in patients undergoing D-P treatment. (C) 2001 Elsevier Science Ltd. Ali rig-hts reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 21:45:39