Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Targeting of high-capacity adenoviral vectors
Autore:
Biermann, V; Volpers, C; Hussmann, S; Stock, A; Kewes, H; Schiedner, G; Herrmann, A; Kochanek, S;
Indirizzi:
Univ Cologne, Ctr Mol Med, D-50931 Cologne, Germany Univ Cologne CologneGermany D-50931 r Mol Med, D-50931 Cologne, Germany Univ Cologne, Inst Genet, D-50931 Cologne, Germany Univ Cologne Cologne Germany D-50931 nst Genet, D-50931 Cologne, Germany Cardion AG, D-40699 Erkrath, Germany Cardion AG Erkrath Germany D-40699Cardion AG, D-40699 Erkrath, Germany
Titolo Testata:
HUMAN GENE THERAPY
fascicolo: 14, volume: 12, anno: 2001,
pagine: 1757 - 1769
SICI:
1043-0342(20010920)12:14<1757:TOHAV>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
VIRAL CODING SEQUENCES; TYPE-5 FIBER PROTEIN; GENE-TRANSFER; MALIGNANT GLIOMA; PENTON BASE; CELL-LINES; RECEPTOR; DELIVERY; TROPISM; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Kochanek, S Univ Cologne, Ctr Mol Med, Kerpener Str 34, D-50931 Cologne, Germany Univ Cologne Kerpener Str 34 Cologne Germany D-50931 Germany
Citazione:
V. Biermann et al., "Targeting of high-capacity adenoviral vectors", HUM GENE TH, 12(14), 2001, pp. 1757-1769

Abstract

High-capacity adenoviral (HC-Ad) vectors contain only the noncoding termini of the viral genome, can deliver large DNA fragments of up to 36 kb into target cells, and feature reduced toxicity and prolonged transgene expression in vivo. To enhance the potential of HC-Ad vectors to transduce specificcell types, we constructed a versatile infectious new helper virus plasmidthat can be used readily to introduce peptide ligands into the III loop ofthe fiber knob domain of Ad5-based HC-Ad vectors. Helper viruses with a 6x-His epitope or Arg-Gly-Asp (RGD) peptide insertion retained the full infectivity of the wild-type helper virus. The RGD-modified helper virus was used for production of a capsid-modified HC-Ad vector expressing beta -galactosidase. The RGD HC-Ad vector transduced the ovarian carcinoma cell lines SK-OV-3 and OVCAR-3 with 4- to 20-fold higher efficiency, compared to unmodified vectors. Transduction of both primary vascular smooth muscle cells as well as primary human endothelial cells was increased up to 15-fold with theRGD-modified vector. Competition experiments with recombinant knob proteinand different RGD peptides indicated that the RGD-mediated transduction was Coxsackie and Adenovirus receptor (CAR)-independent and involved integrinalpha (v)beta (5). The use of fiber-modified helper viruses in the last amplification step of HC-Ad vector production allows for convenient and efficient targeting of these vectors towards different cell types. Targeting strategies will increase the spectrum of applications for HC-Ad vectors and will further add to their safety.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/08/20 alle ore 00:48:07