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Titolo:
Differential expression of cyclooxygenase-2 (COX-2) in human bile duct epithelial cells and bile duct neoplasm
Autore:
Hayashi, N; Yamamoto, H; Hiraoka, N; Dono, K; Ito, Y; Okami, J; Kondo, M; Nagano, H; Umeshita, K; Sakon, M; Matsuura, N; Nakamori, S; Monden, M;
Indirizzi:
Osaka Univ, Grad Sch Med, Dept Surg & Clin Oncol, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 n Oncol, Suita, Osaka 5650871, Japan Osaka Univ, Fac Med, Sch Allied Hlth Sci, Dept Pathol, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 Pathol, Suita, Osaka 5650871, Japan Osaka Sewamens Inst Hosp, Dept Surg, Osaka, Japan Osaka Sewamens Inst Hosp Osaka Japan Inst Hosp, Dept Surg, Osaka, Japan
Titolo Testata:
HEPATOLOGY
fascicolo: 4, volume: 34, anno: 2001,
parte:, 1
pagine: 638 - 650
SICI:
0270-9139(200110)34:4<638:DEOC(I>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRIMARY SCLEROSING CHOLANGITIS; PROSTAGLANDIN G/H SYNTHASE; POLYMERASE-CHAIN-REACTION; COLON-CANCER CELLS; NITRIC-OXIDE; COLORECTAL-CANCER; HEPATOCELLULAR-CARCINOMA; HEMATOGENOUS METASTASIS; PROGNOSTIC FACTORS; NATURAL-HISTORY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
83
Recensione:
Indirizzi per estratti:
Indirizzo: Yamamoto, H Osaka Univ, Grad Sch Med, Dept Surg & Clin Oncol, 2-2 Yamadaoka, Suita, Osaka 5650871, Japan Osaka Univ 2-2 Yamadaoka Suita Osaka Japan 5650871 0871, Japan
Citazione:
N. Hayashi et al., "Differential expression of cyclooxygenase-2 (COX-2) in human bile duct epithelial cells and bile duct neoplasm", HEPATOLOGY, 34(4), 2001, pp. 638-650

Abstract

It is well known that chronic inflammatory conditions involving the bile ducts predispose to the development of bile duct carcinoma, although the relationship between chronic inflammation and malignant transformation is unclear. In this study, by combining immunohistochemistry and computer imaging techniques, we quantified and compared the cyclooxygenase-2 (COX-2) proteinexpression levels of epithelial cells according with their histopathological backgrounds. This technique revealed that the highest levels of COX-2 were expressed in bile duct carcinoma cells, mainly in cytoplasm, and the expression pattern was homogenous and abundant. Moderate levels of COX-2 protein expression were also observed in noncancerous epithelial cells with inflammatory reaction, but the staining intensity was heterogeneous among the positive cells exhibiting inflammation. In contrast, only scattered weak reactivity of COX-2 protein was observed in the noncancerous bile duct epithelial cells without inflammatory reaction. Moreover, bile duct epithelial cells in primary sclerosing cholangitis (PSC) showed very strong expression ofCOX-2 protein, that was comparable with carcinoma cells. On the other hand, primary biliary cirrhosis (PBC) epithelial cells showed moderate levels of COX-2 expression. in addition, specific COX-2 inhibitors, JTE-522 and NS-398, directly inhibited the growth of 4 bile duct carcinoma and I gall bladder carcinoma cell lines that expressed COX-2 protein, in vitro. These datasuggest that COX-2 expression might regulate carcinogenesis of bile duct epithelial cells in inflammatory.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 07:50:48