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Titolo:
Rapid mutational analysis of N-ras proto-oncogene in hematologic malignancies: a study of 77 Greek patients
Autore:
Speletas, M; Arvanitidis, K; Tzoanopoulos, D; Tsironidou, V; Pardali, E; Aggeli, C; Tsapogas, P; Kartalis, G; Sideras, P; Ritis, K;
Indirizzi:
Democritus Univ Thrace, Dept Internal Med 1, Alexandroupolis, Greece Democritus Univ Thrace Alexandroupolis Greece , Alexandroupolis, Greece Papageorgiou Gen Hosp, Dept Hematol, Thessaloniki, Greece Papageorgiou GenHosp Thessaloniki Greece Hematol, Thessaloniki, Greece Umea Univ, Dept Cell & Mol Biol, Umea, Sweden Umea Univ Umea SwedenUmea Univ, Dept Cell & Mol Biol, Umea, Sweden
Titolo Testata:
HAEMATOLOGICA
fascicolo: 9, volume: 86, anno: 2001,
pagine: 918 - 927
SICI:
0390-6078(200109)86:9<918:RMAONP>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACUTE MYELOID-LEUKEMIA; CHRONIC MYELOGENOUS LEUKEMIA; ACUTE LYMPHOBLASTIC-LEUKEMIA; MYELODYSPLASTIC SYNDROMES; POINT MUTATIONS; GENE-MUTATIONS; K-RAS; MOLECULAR PATHOPHYSIOLOGY; PROGNOSTIC-SIGNIFICANCE; CYTOTOXIC THERAPY;
Keywords:
N-ras; acute leukemia; myelodysplastic syndromes; chronic myeloproliferative disorders; NIRCA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Speletas, M Dimarchou K Tsirou 8, Thessaloniki 54248, Greece Dimarchou K Tsirou 8 Thessaloniki Greece 54248 54248, Greece
Citazione:
M. Speletas et al., "Rapid mutational analysis of N-ras proto-oncogene in hematologic malignancies: a study of 77 Greek patients", HAEMATOLOG, 86(9), 2001, pp. 918-927

Abstract

Background and Objectives. N-ras mutations are the most commonly detected molecular abnormalities in hematologic malignancies, especially in those ofmyeloid origin. Different techniques have been used to detect N-ras mutations; however, most of them are either labor intensive or provide sequence data for only a limited number of codons. Consequently, study of the N-ras oncogene has not been convenient in every day clinical practice being restricted, as a rule, to retrospective analysis of patients. Design and Methods. In this study we used a recently developed method thatenables rapid and reliable detection of mutations at the cDNA level; namely, the non-isotopic RNase cleavage assay (NIRCA). Using this method we wereable to screen the N-ras oncogene rapidly and determine the incidence and prognostic significance of N-ras mutations in 77 Greek patients with acute leukemia; myelodysplastic syndromes and chronic myeloproliferative disorders, both. at presentation and during relapse or progression of the disease. Results. Activating N-ras mutations were detected in 7 patients and our results were confirmed by direct sequencing. Interestingly, two novel alterations were identified, a mutation at codon 8 (characterized by a substitution of valine by leucine) in a patient with chronic myeloid leukemia during hematologic relapse of the disease and a polymorphism at codon 92 (1002T -->C, without amino acid substitution) in a patient with chronic myelomonocytic leukemia. Interpretation and Conclusions. A rapid and easy protocol that allows the analysis of N-ras sequences has been developed. This reverse transcription-polymerase chain reaction (RT-PCR)/NIRCA protocol can allow the study of this proto-oncogene in every day clinical practice; rapidly facilitating the validation of the diagnostic and prognostic value of N-ras mutational analyses in patients with hematologic malignancies. (C) 2001, Ferrata Storti Foundation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 12:27:39