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Titolo:
Drosophila synaptotagmin/null mutants survive to early adulthood
Autore:
Loewen, CA; Mackler, JM; Reist, NE;
Indirizzi:
Colorado State Univ, Dept Anat & Neurobiol, Program Mol Cellular & Integrat Neurosci, Ft Collins, CO 80523 USA Colorado State Univ Ft Collins CO USA80523 sci, Ft Collins, CO 80523 USA
Titolo Testata:
GENESIS
fascicolo: 1, volume: 31, anno: 2001,
pagine: 30 - 36
SICI:
1526-954X(200109)31:1<30:DSMSTE>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
SYNAPTIC TRANSMISSION; C2B DOMAIN; SNARE COMPLEX; NEUROTRANSMITTER RELEASE; MEMBRANE-FUSION; BINDING; VESICLES; CA2+; EXOCYTOSIS; LOCALIZATION;
Keywords:
neurotransmission; synapse; exocytosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Reist, NE Colorado State Univ, Dept Anat & Neurobiol, Program Mol Cellular& Integrat Neurosci, Ft Collins, CO 80523 USA Colorado State Univ Ft Collins CO USA 80523 llins, CO 80523 USA
Citazione:
C.A. Loewen et al., "Drosophila synaptotagmin/null mutants survive to early adulthood", GENESIS, 31(1), 2001, pp. 30-36

Abstract

Synaptotagmin is a synaptic vesicle protein required for efficient neurotransmitter release, yet its exact role in the synaptic vesicle cycle is unclear. Drosophila presents an ideal organism for studies aimed at determiningthe in vivo functions of proteins. However, synaptotagmin studies have been limited by the early (embryonic or first instar) lethality previously reported for Drosophila synaptotagmin I null (syt(null)) mutants. Here we report a new culturing technique that enhances survival of severely uncoordinated mutants thereby permitting Drosophila syt(null) mutants to survive through early adulthood. We examined synapses in syt(null) third instar larvae by electrophysiology and found that they exhibit severely decreased and asynchronous evoked neurotransmitter release, as well as an increased rate of spontaneous neurotransmitter release, as previously seen in first instar syt(null) larvae. The ability to examine severe synaptotagmin mutants as thirdinstar larvae, a stage where electrophysiological and morphological analyses are more easily accomplished, will facilitate structure/function studies. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 03:32:11