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Titolo:
Drosophila rab GDI mutants disrupt development but have normal rab membrane extraction
Autore:
Ricard, CS; Jakubowski, JM; Verbsky, JW; Barbieri, MA; Lewis, WM; Fernandez, GE; Vogel, M; Tsou, C; Prasad, V; Stahl, PD; Waksman, G; Cheney, CM;
Indirizzi:
Pomona Coll, Dept Biol, Claremont, CA 91711 USA Pomona Coll Claremont CA USA 91711 ll, Dept Biol, Claremont, CA 91711 USA Washington Univ, Sch Med, Dept Biochem & Mol Biophys, St Louis, MO USA Washington Univ St Louis MO USA Biochem & Mol Biophys, St Louis, MO USA Washington Univ, Sch Med, Dept Genet, St Louis, MO USA Washington Univ StLouis MO USA v, Sch Med, Dept Genet, St Louis, MO USA Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USAWashington Univ St Louis MO USA 63110 l & Physiol, St Louis, MO 63110 USA
Titolo Testata:
GENESIS
fascicolo: 1, volume: 31, anno: 2001,
pagine: 17 - 29
SICI:
1526-954X(200109)31:1<17:DRGMDD>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
GTP-BINDING PROTEIN; NUCLEOTIDE DISSOCIATION INHIBITOR; SMG P25A; REGULATORY PROTEIN; MENTAL-RETARDATION; MOLECULAR-CLONING; IN-VIVO; MELANOGASTER; TRANSPORT; IDENTIFICATION;
Keywords:
rab GDP dissociation inhibitor (GDI); Drosophila; oogenesis; puparium formation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Cheney, CM Pomona Coll, Dept Biol, 609 N Coll Ave, Claremont, CA 91711 USAPomona Coll 609 N Coll Ave Claremont CA USA 91711 CA 91711 USA
Citazione:
C.S. Ricard et al., "Drosophila rab GDI mutants disrupt development but have normal rab membrane extraction", GENESIS, 31(1), 2001, pp. 17-29

Abstract

Rab GTPases are essential for vesicular transport. Rab GDP dissociation inhibitor (GDI) binds to GDP-bound rabs, removes rabs from acceptor membranesand delivers rabs to donor membranes. We isolated lethal GDI mutations in Drosophila and analyzed their developmental phenotypes. To learn how these mutations affect GDI structure, the crystal structure of Drosophila GDI wasdetermined by molecular replacement to a resolution of 3.0 Angstrom. Two hypomorphic, missense mutations are located in domain II of GDI at highly conserved positions, but not in previously identified sequence conserved regions. The mutant GDIs were tested for ability to extract rabs from membranesand showed wild-type levels of rab membrane extraction. The two missense alleles showed intragenic complementation, indicating that domain II of GDI may have two separable functions. This study indicates that GDI function isessential for development of a complex, multicellular organism and that puparium formation and pole cell formation are especially dependent on GDI function. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 21:10:17