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Titolo:
Highly efficient gene transfer into murine liver achieved by intravenous administration of naked Epstein-Barr virus (EBV)-based plasmid vectors
Autore:
Cui, FD; Kishida, T; Ohashi, S; Asada, H; Yasutomi, K; Satoh, E; Kubo, T; Fushiki, S; Imanishi, J; Mazda, O;
Indirizzi:
Kyoto Prefectural Univ Med, Res Inst Neurol Dis & Geriatr, Dept Microbiol,Kyoto 6028566, Japan Kyoto Prefectural Univ Med Kyoto Japan 6028566 biol,Kyoto 6028566, Japan Kyoto Prefectural Univ Med, Res Inst Neurol Dis & Geriatr, Dept Orthoped, Kyoto 6028566, Japan Kyoto Prefectural Univ Med Kyoto Japan 6028566 ped, Kyoto 6028566, Japan Kyoto Prefectural Univ Med, Res Inst Neurol Dis & Geriatr, Dept Pathol & Appl Neurobiol, Kyoto 6028566, Japan Kyoto Prefectural Univ Med Kyoto Japan 6028566 iol, Kyoto 6028566, Japan
Titolo Testata:
GENE THERAPY
fascicolo: 19, volume: 8, anno: 2001,
pagine: 1508 - 1513
SICI:
0969-7128(200110)8:19<1508:HEGTIM>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELLS IN-VIVO; POLYAMIDOAMINE DENDRIMER; TRANSGENE EXPRESSION; EPISOMAL VECTORS; DIRECT-INJECTION; MAMMALIAN-CELLS; HVJ-LIPOSOME; RODENT CELLS; DNA; VITRO;
Keywords:
gene therapy; naked DNA; Epstein-Barr virus-based plasmid vector; episomal vector; hydrodynamics; liver;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Mazda, O Kyoto Prefectural Univ Med, Res Inst Neurol Dis & Geriatr, Dept Microbiol,Kyoto 6028566, Japan Kyoto Prefectural Univ Med Kyoto Japan 6028566 o 6028566, Japan
Citazione:
F.D. Cui et al., "Highly efficient gene transfer into murine liver achieved by intravenous administration of naked Epstein-Barr virus (EBV)-based plasmid vectors", GENE THER, 8(19), 2001, pp. 1508-1513

Abstract

Naked plasmid DNA (pDNA) injection could become an alternative procedure to viral and nonviral gene delivery systems. We have previously shown that Epstein-Barr virus (EBV)-based plasmid vectors containing the EBV nuclear antigen 1 (EBNA 1) gene and the oriP sequence enable quite high and long-lasting expression in various in vitro and in vivo transfection systems. The EBV-based plasmids were intravenously injected into mice via their tail vein under high pressure. A large amount of the marker gene product was expressed in the liver, as much as 320 mug of luciferase was demonstrated per gram of liver at 8 to 24 h after a single injection with 10 mug of DNA. More than 70% of liver cells stained with X-gal when beta -gal gene was transferred. The expression level was significantly higher than that obtained by conventional pDNA lacking the EBNA 1 gene and oriP. On day 35 after the transfection, the expression from the EBV-based plasmid was approximately 100-fold stronger than the conventional pDNA gene expression. Both the EBNA1 gene and oriP are a prerequisite for the augmentation of the transfection efficiency. These results suggest that the intravascular transfection with naked EBV-based plasmid may provide a quite efficient, simple and convenient means to transduce therapeutic genes in vivo into the liver.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 05:17:41