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Titolo:
Activity profiles of bacterioplankton in a eutrophic river
Autore:
Sinsabaugh, RL; Foreman, CM;
Indirizzi:
Univ Toledo, Dept Biol, Toledo, OH 43606 USA Univ Toledo Toledo OH USA 43606 v Toledo, Dept Biol, Toledo, OH 43606 USA
Titolo Testata:
FRESHWATER BIOLOGY
fascicolo: 9, volume: 46, anno: 2001,
pagine: 1239 - 1249
SICI:
0046-5070(200109)46:9<1239:APOBIA>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
DISSOLVED ORGANIC NITROGEN; MICROBIAL COMMUNITIES; MESOTROPHIC LAKE; MARINE WATERS; AMINO-ACIDS; CARBON; DECOMPOSITION; AVAILABILITY; PHOSPHATASES; ECOSYSTEMS;
Keywords:
bacteria; dissolved organic matter; extracellular enzyme activity; river; substrate utilization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Sinsabaugh, RL Univ Toledo, Dept Biol, Toledo, OH 43606 USA Univ Toledo Toledo OH USA 43606 Biol, Toledo, OH 43606 USA
Citazione:
R.L. Sinsabaugh e C.M. Foreman, "Activity profiles of bacterioplankton in a eutrophic river", FRESHW BIOL, 46(9), 2001, pp. 1239-1249

Abstract

1. The significance of microbial diversity in processing dissolved organicmatter (DOM) is largely unknown. We investigated the range of functional diversity in the bacterioplankton from a eutrophic river by profiling extracellular enzyme activities (EEA) and substrate-induced respiration (SIR) patterns.2. The EEA profiles consisted of assays for 21 hydrolases, measured using fluorogenic substrates arrayed on 96-well microplates. Commercially available BiOLOG(R) GN and ECO plates, which contained 120 different substrates, were used for the SIR profiles.3. The EEA data were more dynamic than the SIR. Five enzymes, leucine aminopeptidase, alkaline phosphatase, alanine aminopeptidase, arginine aminopeptidase and P-glucosidase, showed consistently high activity; ten others were ubiquitous at lower activity levels; the remainder were detected intermittently. The SIR data showed less temporal variability. With one exception (citrate), the 20 substrates that generated the largest responses were all saccharides or their derivatives.4. The EEA and SIR data did not generally correlate. Both methods were effective for ordinating bacterioplankton although, unlike the SIR, the EEA ordination followed a clear temporal trajectory.5. Because the SIR profiles are based on a culture response, whilst the EEA profiles measure activity of the extant community, the latter appear to be more directly linked to the mechanics of DOM processing.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 10:53:59