Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Astrocyte-released cytokines induce ramification and outward K+ channel expression in microglia via distinct signalling pathways
Autore:
Schilling, T; Nitsch, R; Heinemann, U; Haas, D; Eder, C;
Indirizzi:
Humboldt Univ, Inst Physiol, D-10117 Berlin, Germany Humboldt Univ Berlin Germany D-10117 st Physiol, D-10117 Berlin, Germany Humboldt Univ, Inst Anat, D-10098 Berlin, Germany Humboldt Univ Berlin Germany D-10098 Inst Anat, D-10098 Berlin, Germany
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 3, volume: 14, anno: 2001,
pagine: 463 - 473
SICI:
0953-816X(200108)14:3<463:ACIRAO>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; CENTRAL-NERVOUS-SYSTEM; GROWTH-FACTOR-BETA; CLASS-II TRANSACTIVATOR; MHC GENE-EXPRESSION; PROTEIN-KINASE-C; TGF-BETA; IN-VITRO; TRANSFORMING GROWTH-FACTOR-BETA-1; ELECTROPHYSIOLOGICAL PROPERTIES;
Keywords:
brain macrophages; granulocyte/macrophage colony-stimulating factor; macrophage colony-stimulating factor; mouse; transforming growth factor-beta;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Eder, C Humboldt Univ, Inst Physiol, Tucholskystr 2, D-10117 Berlin, Germany Humboldt Univ Tucholskystr 2 Berlin Germany D-10117 rlin, Germany
Citazione:
T. Schilling et al., "Astrocyte-released cytokines induce ramification and outward K+ channel expression in microglia via distinct signalling pathways", EUR J NEURO, 14(3), 2001, pp. 463-473

Abstract

Differentiation of microglial cells is characterized by transformation from ameboid into ramified cell shape and up-regulation of K+ channels. The processes of microglial differentiation are controlled by astrocytic factors. The mechanisms by which astrocytes cause developmental changes in morphological and electrophysiological properties of microglia have remained unclear. We show here that the cytokines transforming growth factor-beta (TGF-beta), macrophage colony-stimulating factor (M-CSF) and granulocyte/macrophagecolony-stimulating factor (GM-CSF) are released by astrocytes at concentrations sufficient to induce ramification and up-regulation of delayed rectifier (DR) K+ channels in microglia. Transformation from ameboid into ramified morphology induced in microglia by exposure to astrocyte-conditioned medium (ACM) was inhibited by neutralizing antibodies against TGF-beta, M-CSF or GM-CSF, whilst ACM-induced DR channel expression was exclusively inhibited by antibodies against TGF-beta. Although both ramification and DR channelup-regulation occurred simultaneously, DR channel blockade by charybdotoxin failed to inhibit microglial ramification. The ACM-induced ramification of microglia was inhibited by the tyrosine kinase inhibitor genistein, whereas DR channel up-regulation did not occur in the presence of the serine/threonine kinase inhibitor H7. Our data suggest that astrocytes modulate processes of microglial differentiation in parallel but via distinct signalling pathways.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 23:06:37