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Titolo:
Identification of corticosteroid-responsive genes in rat hippocampus usingserial analysis of gene expression
Autore:
Datson, NA; van der Perk, J; de Kloet, ER; Vreugdenhil, E;
Indirizzi:
Leiden Univ, Med Ctr, Leiden Amsterdam Ctr Drug Res, Div Med Pharmacol, NL-2300 RA Leiden, Netherlands Leiden Univ Leiden Netherlands NL-2300 RA NL-2300 RA Leiden, Netherlands
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 4, volume: 14, anno: 2001,
pagine: 675 - 689
SICI:
0953-816X(200108)14:4<675:IOCGIR>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
INHIBIT GLUCOSE-TRANSPORT; MESSENGER-RNA EXPRESSION; GLUCOCORTICOID RECEPTOR; MINERALOCORTICOID RECEPTOR; CA1 NEURONS; BRAIN; ADRENALECTOMY; STRESS; CELLS; CONDUCTANCES;
Keywords:
corticosterone; expression profiling; glucocorticoid receptor; mineralocorticoid receptor; neuronal plasticity; SAGE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Datson, NA Leiden Univ, Med Ctr, Leiden Amsterdam Ctr Drug Res, Div Med Pharmacol, NL-2300 RA Leiden, Netherlands Leiden Univ Leiden Netherlands NL-2300 RA Leiden, Netherlands
Citazione:
N.A. Datson et al., "Identification of corticosteroid-responsive genes in rat hippocampus usingserial analysis of gene expression", EUR J NEURO, 14(4), 2001, pp. 675-689

Abstract

Adrenal corticosteroids (CORT) have a profound effect on the function of the hippocampus. This is mediated in a coordinated manner by mineralocorticoid (MR) and glucocorticoid receptors (GR) via activation or repression of target genes. The aim of this study was to identify, using serial analysis of gene expression (SAGE), CORT-responsive hippocampal genes regulated via MR and/or GR. SAGE profiles were compared under different conditions of CORTexposure, resulting in the identification of 203 CORT-responsive genes that are involved in many different cellular processes like, energy expenditure and cellular metabolism; protein synthesis and turnover; signal transduction and neuronal connectivity and neurotransmission. Besides some previously identified CORT-responsive genes, the majority of the genes identified inthis study were novel. In situ hybridization revealed that six randomly chosen CORT-responsive genes had distinct expression patterns in neurons of the hippocampus. In addition, using in situ hybridization, we confirmed thatthese six genes were indeed regulated by CORT, underscoring the validity of the SAGE data. Comparison of MR- and GR-dependent expression profiles revealed that the majority of the CORT-responsive genes were regulated either by activated MR or by activated GR, while only a few genes were responsive to both activated MR and GR. This indicates that the molecular basis for the differential effects of activated MR and GR is activation or repression of distinct, yet partially overlapping sets of genes. The putative CORT-responsive genes identified here will provide insight into the molecular mechanisms underlying the differential and sometimes opposing effects of MR and GR on neuronal excitability, memory formation and behaviour as well as theirrole in neuronal protection and damage.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/12/18 alle ore 23:32:20