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Titolo:
Two distinct types of repetitive bursting activity mediated by NMDA in hypothalamic neurons in vitro
Autore:
Poulain, P;
Indirizzi:
INSERM, U422, F-59045 Lille, France INSERM Lille France F-59045INSERM, U422, F-59045 Lille, France
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 4, volume: 14, anno: 2001,
pagine: 657 - 665
SICI:
0953-816X(200108)14:4<657:TDTORB>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
METHYL-D-ASPARTATE; MAGNOCELLULAR DORSAL NUCLEUS; GUINEA-PIG HYPOTHALAMUS; RAT SPINAL-CORD; INDUCED OSCILLATIONS; IN-VITRO; VOLTAGE OSCILLATIONS; DOPAMINE NEURONS; BASALIS NEURONS; LATERAL SEPTUM;
Keywords:
guinea pig; low-threshold spike; slice; whole-cell;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Poulain, P INSERM, U422, Pl Verdun, F-59045 Lille, France INSERM Pl VerdunLille France F-59045 n, F-59045 Lille, France
Citazione:
P. Poulain, "Two distinct types of repetitive bursting activity mediated by NMDA in hypothalamic neurons in vitro", EUR J NEURO, 14(4), 2001, pp. 657-665

Abstract

Hypothalamic magnocellular dorsal nucleus neurons were recorded from adultguinea pig brain slices with the whole-cell patch-clamp technique to determine the effects of N-methyl-D-aspartate (NMDA) applied in the bath or by iontophoresis. In a majority of cells (59 of 77, 76.6%), rhythmic bursting discharges were evoked by specific activation of NMDA receptors when the membrane was more negative than -60 mV. This endogenous rhythmic activity was resistant to tetrodotoxin. It was suppressed by removal of extracellular Mg2+, indicating the involvement of the voltage-dependent block of the NMDA channel by Mg2+. Application of thapsigargin showed that rhythmic activity did not depend on the release of Ca2+ from reticulum stores. Blockers of Ca2 conductances Ni2+ and nifedipine had no effects on the bursts. Their repolarization did not involve the activation of a strophantidin- or ouabain-sensitive pump, but partly depended on an apamine-sensitive Ca2+-dependent K()current. In a small subset of cells (9 of 69, 13%), specific activation of NMDA receptors induced another type of bursting activity which consisted of repetitive low-threshold spikes sustaining bursts of action potentials. Rhythmic low-threshold spikes subsisted in the presence of tetrodotoxin butwere suppressed by Ni2+. Increasing the amount of NMDA brought about a switch from the rhythmic low-threshold spike burst firing to the rhythmic bursting activity observed for the majority of cells. The present data show forthe first time that NMDA receptor activation can induce two independent rhythmic bursting behaviours in the same neuron, probably depending on the strength of the glutamatergic drive.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 13:06:13