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Titolo:
Neuronal nitric oxide synthase (nNOS) mRNA is downregulated, and constitutive NOS enzymatic activity decreased, in thoracic dorsal root ganglia and spinal cord of the rat by a substance PN-terminal metabolite
Autore:
Kovacs, KJ; Cai, YJ; Larson, AA;
Indirizzi:
Univ Minnesota, Dept Vet Pathobiol, St Paul, MN 55108 USA Univ Minnesota St Paul MN USA 55108 Vet Pathobiol, St Paul, MN 55108 USA
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 4, volume: 14, anno: 2001,
pagine: 577 - 584
SICI:
0953-816X(200108)14:4<577:NNOS(M>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXHIBITS ANTINOCICEPTIVE ACTIVITY; PRIMARY SENSORY NEURONS; CENTRAL-NERVOUS-SYSTEM; GENE-RELATED PEPTIDE; METHYL-D-ASPARTATE; MESSENGER-RNA; INTERSTITIAL CYSTITIS; INCREASED EXPRESSION; PERIPHERAL AXOTOMY; NEUROPATHIC PAIN;
Keywords:
arginine; citrulline; hyperalgesia; pain; writhing;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
77
Recensione:
Indirizzi per estratti:
Indirizzo: Larson, AA Univ Minnesota, Dept Vet Pathobiol, 295 Anim Sci Vet Med Bldg,1988 Fitch Ave, St Paul, MN 55108 USA Univ Minnesota 295 Anim Sci Vet Med Bldg,1988 Fitch Ave St Paul MN USA 55108
Citazione:
K.J. Kovacs et al., "Neuronal nitric oxide synthase (nNOS) mRNA is downregulated, and constitutive NOS enzymatic activity decreased, in thoracic dorsal root ganglia and spinal cord of the rat by a substance PN-terminal metabolite", EUR J NEURO, 14(4), 2001, pp. 577-584

Abstract

Nitric oxide (NO) in the spinal cord plays a role in sensory and autonomicactivity. Pain induced by acetic acid in the abdominal stretch (writhing) assay and hyperalgesia associated with chronic pain are highly sensitive toNO synthase (NOS) inhibitors. Because substance P (SP) is released and up-regulated in some models of chronic pain, we hypothesized that an accumulation of SP metabolites may influence NOS expression and activity. To test this hypothesis, we examined the effect of intrathecally (i.t.) injected substance P (1-7) [SP(1-7)], the major metabolite of SP in the rat, on neuronalNOS (nNOS) mRNA in the thoracic and lumbar spinal cord, dorsal root ganglia (DRG) and on the corresponding constitutive NOS (cNOS) enzyme activity. Detected using quantitative RT-PCR, nNOS mRNA content in the thoracic spinalcord was decreased 6 h after injection of 5 mu mol of SP(1-7) and returnedto control 2 days later. In thoracic DRG, nNOS mRNA was reduced 48 h afterSP(1-7). The cNOS enzymatic activity in thoracic spinal tissue was gradually decreased to a minimum at 72 h. Down-regulation of NOS by SP(1-7) in thethoracic area appears to be highly associated with capsaicin-sensitive primary afferent neurons. No similar changes in either parameter were measuredin the lumbar area after SP(1-7). These data suggest that N-terminal SP fragments, which are known to cause long-term antinociception in the writhingassay, may do so by their ability to down-regulate NO synthesis along nociceptive pathways.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 06:48:41