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Titolo:
The antitumour agent 5,6-dimethylxanthenone-4-acetic acid acts in vitro onhuman mononuclear cells as a co-stimulator with other inducers of tumour necrosis factor
Autore:
Philpott, M; Ching, LM; Baguley, BC;
Indirizzi:
Univ Auckland, Sch Med, Auckland Canc Soc, Res Ctr, Auckland, New Zealand Univ Auckland Auckland New Zealand Soc, Res Ctr, Auckland, New Zealand
Titolo Testata:
EUROPEAN JOURNAL OF CANCER
fascicolo: 15, volume: 37, anno: 2001,
pagine: 1930 - 1937
SICI:
0959-8049(200110)37:15<1930:TAA5AA>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
NF-KAPPA-B; FLAVONE ACETIC-ACID; FACTOR-ALPHA PRODUCTION; BINDING-PROTEIN; HUMAN-MONOCYTES; KINASE; LIPOPOLYSACCHARIDE; INDUCTION; INHIBITION; PHOSPHORYLATION;
Keywords:
lipopolysaccharide; CD14; interleukin-1; okadaic acid; phorbol ester; NF-kappaB;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Baguley, BC Univ Auckland, Sch Med, Auckland Canc Soc, Res Ctr, Auckland, New Zealand Univ Auckland Auckland New Zealand r, Auckland, New Zealand
Citazione:
M. Philpott et al., "The antitumour agent 5,6-dimethylxanthenone-4-acetic acid acts in vitro onhuman mononuclear cells as a co-stimulator with other inducers of tumour necrosis factor", EUR J CANC, 37(15), 2001, pp. 1930-1937

Abstract

5,6-Dimethylxanthenone-4-acetic acid (DMXAA), currently in phase I trials,demonstrates excellent activity against transplantable murine tumours withestablished vasculature. The induction of cytokines, particularly of tumour necrosis factor (TNF), appears to be critical to its action. We investigated TNF induction by DMXAA in cultured human peripheral blood leucocytes (HPBL). TNF was measured by an enzyme-linked immunosorbent assay after 8 h, and NF-kappaB induction by electrophoretic mobility shift assays (EMSA) after 2 h. DMXAA (800 mug/ml) had no effect alone on TNF production but augmented, by up to 4-fold, the ability of bacterial lipopolysaccharide (LPS) to induce TNF. Previously reported results showing TNF production by DMXAA alone were traced to the presence in an earlier batch of DMXAA of a small amount of LPS, the action of which could be blocked by polymyxin B. DMXAA stimulated TNF production by deacylated LPS, which alone had little effect. An antibody (MEM-18) to the CD14 receptor, while blocking the induction of TNF by LPS, enabled DMXAA to both synthesise TNF and induce NF-kappaB. The structurally related drug, flavone acetic acid (FAA), did not induce TNF or synergise with anti-CD14 antibody. DMXAA strongly augmented the ability of suboptimal concentrations of interleukin-1 (IL-1) (25 ng/ml), okadaic acid (OA)(20 ng/ml) and phorbol-12-myristate-13-acetate (PMA) (5 ng/ml) to induce TNF production, suggesting that it affects multiple pathways converging on NF-kappaB activation. Sodium salicylate, a drug reported to inhibit the beta-subunit of I kappaB kinase (IKK), appeared to competitively inhibit TNF production by DMXAA in the presence of anti-CD14 antibody. Taken together, the results indicate DMXAA acts in vitro on HPBL to co-stimulate TNF production by a wide variety of agents, and suggests that IKK is the target that mediates this action. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 21:53:54