Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Fluid phase and receptor-mediated endocytosis in Paramecium primaurelia byfluorescence confocal laser scanning microscopy
Autore:
Ramoino, P; Fronte, P; Fato, M; Beltrame, F; Robello, M; Diaspro, A;
Indirizzi:
Univ Genoa, DIP TE RIS, I-16132 Genoa, Italy Univ Genoa Genoa Italy I-16132 v Genoa, DIP TE RIS, I-16132 Genoa, Italy Univ Genoa, Dept Phys, I-16146 Genoa, Italy Univ Genoa Genoa Italy I-16146 iv Genoa, Dept Phys, I-16146 Genoa, Italy Univ Genoa, INFM, I-16146 Genoa, Italy Univ Genoa Genoa Italy I-16146Univ Genoa, INFM, I-16146 Genoa, Italy Univ Genoa, DIST, I-16145 Genoa, Italy Univ Genoa Genoa Italy I-16145Univ Genoa, DIST, I-16145 Genoa, Italy
Titolo Testata:
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
fascicolo: 5, volume: 30, anno: 2001,
pagine: 305 - 312
SICI:
0175-7571(200109)30:5<305:FPAREI>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
COATED PIT FORMATION; INTRACELLULAR-TRANSPORT; POTASSIUM-DEPLETION; HYPERTONIC MEDIUM; RAT HEPATOCYTES; EARLY ENDOSOMES; LECTIN-BINDING; CLATHRIN; PHAGOCYTOSIS; ACIDIFICATION;
Keywords:
WGA; dextran; clathrin inhibitors; fluorescence CLSM; ciliated protozoa;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Ramoino, P Univ Genoa, DIP TE RIS, Corso Europa 26, I-16132 Genoa, Italy Univ Genoa Corso Europa 26 Genoa Italy I-16132 32 Genoa, Italy
Citazione:
P. Ramoino et al., "Fluid phase and receptor-mediated endocytosis in Paramecium primaurelia byfluorescence confocal laser scanning microscopy", EUR BIOPHYS, 30(5), 2001, pp. 305-312

Abstract

In ciliated protozoa, most nutrients are internalized via phagocytosis by food vacuole formation at the posterior end of the buccal cavity. The uptake of small-sized molecules and external fluid through the plasma membrane is a localized process. That is because most of the cell surface is internally covered by an alveolar system and a fibrous epiplasm, so that only defined areas of the cell surface are potential substance uptake sites. The purpose of this study is to analyze, by fluorescence confocal laser scanning microscopy, the relationship between WGA (Triticum vulgaris agglutinin) and dextran internalization in Paramecium primaurelia cells blocked in the phagocytic process, so that markers could not be internalized via food vacuole formation. WGA, which binds to surface constituents of fixed and living cells, was used as a marker for membrane transport and dextran as a marker for fluid phase endocytosis. After 3 min incubation, WGA-FITC is found on plasma membrane and cilia, and successively within small cytoplasmic vesicles. After a 10-15 min chase in unlabeled medium, the marked vesicles decrease innumber, increase in size and fuse with food vacuoles. This fusion was evidenced by labeling food vacuoles with BSA-Texas red. Dextran enters the cellvia endocytic vesicles which first localize in the cortical region, under the plasma membrane, and then migrate in the cytoplasm and fuse with other endocytic vesicles and food vacuoles. When cells are fed with WGA-FITC and dextran-Texas red at the same time, two differently labeled vesicle populations are found. Cytosol acidification and incubation in sucrose medium or in chlorpromazine showed that WGA is internalized via clathrin vesicles, whereas fluid phase endocytosis is a clathrin-independent process.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 05:39:27