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Titolo:
Infertility and testicular defects in hormone-sensitive lipase-deficient mice
Autore:
Chung, SR; Wang, SP; Pan, LG; Mitchell, G; Trasler, J; Hermo, L;
Indirizzi:
McGill Univ, Montreal Childrens Hosp, Res Inst, Montreal, PQ H3H 1P3, Canada McGill Univ Montreal PQ Canada H3H 1P3 Inst, Montreal, PQ H3H 1P3, Canada McGill Univ, Dept Pediat, Montreal, PQ H3H 1P3, Canada McGill Univ Montreal PQ Canada H3H 1P3 diat, Montreal, PQ H3H 1P3, Canada McGill Univ, Dept Human Genet, Montreal, PQ H3H 1P3, Canada McGill Univ Montreal PQ Canada H3H 1P3 enet, Montreal, PQ H3H 1P3, Canada McGill Univ, Dept Pharmacol & Therapeut, Montreal, PQ H3H 1P3, Canada McGill Univ Montreal PQ Canada H3H 1P3 peut, Montreal, PQ H3H 1P3, Canada Hop St Justine, Res Ctr, Dept Pediat, Montreal, PQ H3T 1C5, Canada Hop St Justine Montreal PQ Canada H3T 1C5 t, Montreal, PQ H3T 1C5, Canada McGill Univ, Dept Anat, Montreal, PQ H3T 1C5, Canada McGill Univ MontrealPQ Canada H3T 1C5 Anat, Montreal, PQ H3T 1C5, Canada McGill Univ, Dept Cell Biol, Montreal, PQ H3T 1C5, Canada McGill Univ Montreal PQ Canada H3T 1C5 Biol, Montreal, PQ H3T 1C5, Canada
Titolo Testata:
ENDOCRINOLOGY
fascicolo: 10, volume: 142, anno: 2001,
pagine: 4272 - 4281
SICI:
0013-7227(200110)142:10<4272:IATDIH>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHOLESTEROL ESTER HYDROLASE; INTERCELLULAR BRIDGES; ADIPOSE-TISSUE; LIPID-METABOLISM; RAT; EPIDIDYMIS; EXPRESSION; ENZYME; SPERMATOGENESIS; LOCALIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Trasler, J McGill Univ, Montreal Childrens Hosp, Res Inst, 2300 Tupper St,Montreal, PQ H3H 1P3, Canada McGill Univ 2300 Tupper St Montreal PQ CanadaH3H 1P3 3, Canada
Citazione:
S.R. Chung et al., "Infertility and testicular defects in hormone-sensitive lipase-deficient mice", ENDOCRINOL, 142(10), 2001, pp. 4272-4281

Abstract

The 84-kDa hormone-sensitive lipase (gene designation Lipe; EC 3.1.1.3) isa cholesterol esterase and triglyceride hydrolase that functions in the release of fatty acids from adipocytes. The role of hormone-sensitive lipase in other tissues such as the testis, where a specific 120-kDa testis-specific isoform is expressed, is unknown. To study this, we examined the fertility and testicular histology of gene-targeted hormone-sensitive lipase-deficient mice. Homozygous hormone-sensitive lipase-deficient male mice are infertile and have decreased testis weights; female homozygotes are fertile. Testicular abnormalities, detected at the fight and electron microscopic levels, included the presence of multinucleated round and elongating spermatids, vacuolization of the seminiferous epithelium, asynchronization of the spermatogenic cycle, sloughing of postmeiotic germ cells from the seminiferousepithelium into the lumen, and a marked reduction in the numbers of late spermatids. Extensive nuclear head deformation was noted in late spermatids as well as the sharing of a common acrosome in multinucleated cells. In some multinucleated cells, nuclei were separated from their acrosomes, with the acrosomes remaining attached to areas of ectoplasmic specializations, suggesting defects in intercellular cytoplasmic bridge integrity. Although thelumen of the epididymis was essentially devoid of spermatozoa and filled instead with spherical degenerating cells, the epididymal epithelial cells appeared normal. The few late spermatids present in the epididymis were abnormal. There was no morphological evidence, as judged by the absence of lipid droplets of triacylglycerol or cholesteryl ester accumulation in the testis. Together, the data suggest that hormone-sensitive lipase deficiency results in abnormalities in spermiogenesis that are incompatible with normal fertility. We speculate that a metabolite downstream from the hormone sensitive lipase reaction may be essential for membrane stabilization and integrity in the seminiferous epithelium and, in particular, may play an importantrole in the maintenance of intercellular cytoplasmic bridges between postmeiotic germ cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 18:54:02