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Titolo:
Impact of the six nucleotides downstream of the stop codon on translation termination
Autore:
Namy, O; Hatin, I; Rousset, JP;
Indirizzi:
Univ Paris 11, Inst Genet & Microbiol, Lab Genet Mol Traduct, F-91405 Orsay, France Univ Paris 11 Orsay France F-91405 et Mol Traduct, F-91405 Orsay, France
Titolo Testata:
EMBO REPORTS
fascicolo: 9, volume: 2, anno: 2001,
pagine: 787 - 793
SICI:
1469-221X(200109)2:9<787:IOTSND>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI; RIBOSOMAL-RNA; SACCHAROMYCES-CEREVISIAE; YEAST; SIGNAL; EFFICIENCY; CONTEXT; MUTATIONS; TRANSTERM; DATABASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Rousset, JP Univ Paris 11, Inst Genet & Microbiol, Lab Genet Mol Traduct, F-91405 Orsay, France Univ Paris 11 Orsay France F-91405 ct, F-91405 Orsay, France
Citazione:
O. Namy et al., "Impact of the six nucleotides downstream of the stop codon on translation termination", EMBO REP, 2(9), 2001, pp. 787-793

Abstract

The efficiency of translation termination is influenced by local contexts surrounding stop codons. In Saccharomyces cerevisiae, upstream and downstream sequences act synergistically to influence the translation termination efficiency. By analysing derivatives of a leaky stop codon context, we initially demonstrated that at least six nucleotides after the stop codon are a key determinant of readthrough efficiency in S. cerevisiae. We then developed a combinatorial-based strategy to identify poor 3 ' termination contexts. By screening a degenerate oligonucleotide library, we identified a consensus sequence -CA(A/G)N(U/C/G)A-, which promotes >5% readthrough efficiency when located downstream of a UAG stop codon. Potential base pairing betweenthis stimulatory motif and regions close to helix 18 and 44 of the 18S rRNA provides a model for the effect of the 3 ' stop codon context on translation termination.

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Documento generato il 03/06/20 alle ore 00:04:19