Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
A plant viral "reinitiation" factor interacts with the host translational machinery
Autore:
Park, HS; Himmelbach, A; Browning, KS; Hohn, T; Ryabova, LA;
Indirizzi:
Friedrich Miescher Inst, CH-4002 Basel, Switzerland Friedrich Miescher Inst Basel Switzerland CH-4002 002 Basel, Switzerland Univ Texas, Dept Chem & Biochem, Austin, TX 78712 USA Univ Texas Austin TX USA 78712 Dept Chem & Biochem, Austin, TX 78712 USA Univ Texas, Inst Mol & Cellular Biol, Austin, TX 78712 USA Univ Texas Austin TX USA 78712 Mol & Cellular Biol, Austin, TX 78712 USA
Titolo Testata:
CELL
fascicolo: 6, volume: 106, anno: 2001,
pagine: 723 - 733
SICI:
0092-8674(20010921)106:6<723:APV"FI>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
CAULIFLOWER MOSAIC-VIRUS; OPEN-READING-FRAME; POLYCISTRONIC MESSENGER-RNA; INITIATION FACTOR-III; SUBUNIT PROTEIN L18; RIBOSOMAL-SUBUNIT; SACCHAROMYCES-CEREVISIAE; EUKARYOTIC RIBOSOMES; GENE-EXPRESSION; INCLUSION BODY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Hohn, T Friedrich Miescher Inst, POB 2543, CH-4002 Basel, Switzerland Friedrich Miescher Inst POB 2543 Basel Switzerland CH-4002 erland
Citazione:
H.S. Park et al., "A plant viral "reinitiation" factor interacts with the host translational machinery", CELL, 106(6), 2001, pp. 723-733

Abstract

The cauliflower mosaic virus transactivator, TAV, controls translation reinitiation of major open reading frames on polycistronic RNA. We show here that TAV function depends on its association with polysomes and eukaryotic initiation factor eIF3 in vitro and in vivo. TAV physically interacts with eIF3 and the 60S ribosomal subunit. Two proteins mediating these interactions were identified: eIF3g and 60S ribosomal protein L24. Transient expression of eIF3g and L24 in plant protoplasts strongly affects TAV-mediated reinitiation activity. We demonstrate that TAV/eIF3/40S and eIF3/TAV160S ternarycomplexes form in vitro, and propose that TAV mediates efficient recruitment of eIF3 to polysomes, allowing translation of polycistronic mRNAs by reinitiation, overcoming the normal cell barriers to this process.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 12:44:51