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Titolo:
Engineering mammalian cells for solid-state sensor applications
Autore:
Bloom, FR; Price, P; Lao, GF; Xia, JL; Crowe, JH; Battista, JR; Helm, RF; Slaughter, S; Potts, M;
Indirizzi:
Virginia Tech, Ctr Genomics, Blacksburg, VA 24061 USA Virginia Tech Blacksburg VA USA 24061 Genomics, Blacksburg, VA 24061 USA Life Technol, Rockville, MD 20850 USA Life Technol Rockville MD USA 20850Life Technol, Rockville, MD 20850 USA Life Technol, Grand Isl, NY 14072 USA Life Technol Grand Isl NY USA 14072Life Technol, Grand Isl, NY 14072 USA Univ Calif Davis, Dept Mol & Cell Biol, Davis, CA 95616 USA Univ Calif Davis Davis CA USA 95616 Mol & Cell Biol, Davis, CA 95616 USA Louisiana State Univ, Dept Biol Sci, Baton Rouge, LA 70803 USA Louisiana State Univ Baton Rouge LA USA 70803 , Baton Rouge, LA 70803 USA
Titolo Testata:
BIOSENSORS & BIOELECTRONICS
fascicolo: 7-8, volume: 16, anno: 2001,
pagine: 603 - 608
SICI:
0956-5663(200109)16:7-8<603:EMCFSS>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
NOSTOC COMMUNE CYANOBACTERIA; DESICCATION TOLERANCE; POLYSACCHARIDE; PROKARYOTES; PROTEINS;
Keywords:
desiccation tolerance; cyanobacteria; sucrose; anhydrophile; hydrophilin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Potts, M Virginia Tech, Ctr Genomics, Blacksburg, VA 24061 USA Virginia Tech Blacksburg VA USA 24061 , Blacksburg, VA 24061 USA
Citazione:
F.R. Bloom et al., "Engineering mammalian cells for solid-state sensor applications", BIOSENS BIO, 16(7-8), 2001, pp. 603-608

Abstract

A fundamental advance in the development and application of cell- and tissue-based biosensors would be the ability to achieve air-dry stabilization of mammalian (especially human) cells with subsequent recovery following rehydration. The would allow for the preparation of sensors with extended shelf lives, only requiring the addition of water for activation. By understanding and subsequently employing the tactics used by desiccation-tolerant extremophiles, it may be possible to design stabilized mammalian cell-based biosensors. The approaches required to realize this goal are discussed and illustrated with several examples. (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 13/07/20 alle ore 07:37:21