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Titolo:
A two-cell biosensor that couples neuronal cells to optically monitored fish chromatophores
Autore:
Preston, RR; McFadden, PN;
Indirizzi:
Oregon State Univ, Dept Biochem & Biophys, Corvallis, OR 97331 USA Oregon State Univ Corvallis OR USA 97331 Biophys, Corvallis, OR 97331 USA
Titolo Testata:
BIOSENSORS & BIOELECTRONICS
fascicolo: 7-8, volume: 16, anno: 2001,
pagine: 447 - 455
SICI:
0956-5663(200109)16:7-8<447:ATBTCN>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDIVIDUAL CHROMAFFIN CELLS; PC12 CELLS; NEUROTRANSMITTER RELEASE; PHEOCHROMOCYTOMA CELLS; ALPHA-LATROTOXIN; CLONAL LINE; RECEPTOR; CATECHOLAMINES; MELANOPHORES; EXOCYTOSIS;
Keywords:
cytosensor; chromatophore; erythrophore; PC12; neurotransmitter; norepinephrine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: McFadden, PN Oregon State Univ, Dept Biochem & Biophys, Corvallis, OR 97331 USA Oregon State Univ Corvallis OR USA 97331 allis, OR 97331 USA
Citazione:
R.R. Preston e P.N. McFadden, "A two-cell biosensor that couples neuronal cells to optically monitored fish chromatophores", BIOSENS BIO, 16(7-8), 2001, pp. 447-455

Abstract

A two-cell biosensor was developed that uses optically detected changes innaturally colored fish chromatophores to measure the neurosecretory outputof mammalian neuronal cells. The specific version of the biosensor described here is a continuous flow device that places red-pigmented, dendritic erythrophore cells directly downstream of an immobilized population of PC12 neuronal cells, a well-established model cell-line having neuroendocrine function. Agents known to stimulate catecholamine neurosecretion (secretagogues) were presented to the PC12 cells. It was found that the varying level ofneurosecretion from the PC12 cells was measurable by judging the degree ofpigment aggregation in the erythrophores. Increases in catecholamine secretion and consequent pigment aggregation were observed for several known secretagogues, including receptor agonists (ATP, acetylcholine), membrane depolarizing agents (high K+ concentration), and specific neurotoxins (black widow spider venom, alpha -latrotoxin). This particular two-cell biosensor, which is applicable to the detection of any agents that affect the levels ofcatecholamine secretion from PC12 cells, demonstrates the general principle that the breadth of sensitivity of a biosensor is increased by employing coupled cell types. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 03:01:50