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Titolo:
Synthesis and distribution of glycosaminoglycans in human leukemic B- and T-cells and monocytes studied using specific enzymic treatments and high-performance liquid chromatography
Autore:
Makatsori, E; Karamanos, NK; Papadogiannakis, N; Hjerpe, A; Anastassiou, ED; Tsegenidis, T;
Indirizzi:
Univ Patras, Dept Chem, Sect Organ Chem Biochem & Nat PRod, GR-26110 Patras, Greece Univ Patras Patras Greece GR-26110 m & Nat PRod, GR-26110 Patras, Greece Huddinge Univ Hosp, Karolinska Inst, Div Pathol, Dept IMPI, S-14186 Huddinge, Sweden Huddinge Univ Hosp Huddinge Sweden S-14186 MPI, S-14186 Huddinge, Sweden Univ Patras, Sch Med, Dept Microbiol, GR-26110 Patras, Greece Univ PatrasPatras Greece GR-26110 pt Microbiol, GR-26110 Patras, Greece
Titolo Testata:
BIOMEDICAL CHROMATOGRAPHY
fascicolo: 6, volume: 15, anno: 2001,
pagine: 413 - 417
SICI:
0269-3879(200110)15:6<413:SADOGI>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-MALIGNANT MESOTHELIOMA; CHONDROITIN SULFATE PROTEOGLYCAN; HEPARAN-SULFATE; GROWTH-FACTORS; LYMPHOCYTES-T; BIOSYNTHESIS; DIFFERENTIATION; LINE; ACTIVATION; STIMULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Tsegenidis, T Univ Patras, Dept Chem, Sect Organ Chem Biochem & Nat PRod, GR-26110 Patras, Greece Univ Patras Patras Greece GR-26110 GR-26110 Patras, Greece
Citazione:
E. Makatsori et al., "Synthesis and distribution of glycosaminoglycans in human leukemic B- and T-cells and monocytes studied using specific enzymic treatments and high-performance liquid chromatography", BIOMED CHRO, 15(6), 2001, pp. 413-417

Abstract

Identification of glycosaminoglycans (GAGs) synthesized by three human leukaemic cell lines-Jurkat (T-cell leukaemia), Daudi (Burkitt's lymphoma, B-cell leukaemia) and THP-1 (acute monocytic leukemia)-and normal peripheral blood mononuclear cells (PBMC) and their distribution among cell membrane and culture medium were studied. GAGs were isolated using ion-exchange chromatography on DEAE-Sephacel and their composition and fine chemical structurewere studied using highperformance liquid chromatography with radiochemical detection. All cell lines synthesize chondroitin sulphate (CS) and heparan sulphate (HS) in both cell membrane and culture medium. No hyaluronan wasdetected using treatment with specific lyases and highly sensitive HPLC methodology. CS is the major secreted GAG in all cell lines tested and the major cell retained GAG in Jurkat and Daudi. HS is the major GAG in the cell membrane of THP-1. The amounts of distinct GAGs synthesized by all cancer cell lines differ from those produced by normal PBML indicating a major roleof GAGs in malignant transformation of human lymphocytes and monocytes. Copyright (C) 2001 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 10:06:51