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Titolo:
High performance liquid chromatography with UV detection for the simultaneous determination of sympathomimetic amines using 4-(4,5-diphenyl-1H-imidazole-2-yl)benzoyl chloride as a label
Autore:
Kaddoumi, A; Kubota, A; Nakashima, MN; Takahashi, M; Nakashima, K;
Indirizzi:
Nagasaki Univ, Grad Sch Pharmaceut Sci, Dept Analyt Res Pharmacoinformat, Nagasaki 852, Japan Nagasaki Univ Nagasaki Japan 852 s Pharmacoinformat, Nagasaki 852, Japan
Titolo Testata:
BIOMEDICAL CHROMATOGRAPHY
fascicolo: 6, volume: 15, anno: 2001,
pagine: 379 - 388
SICI:
0269-3879(200110)15:6<379:HPLCWU>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
MASS-SPECTROMETRY; D-NORFENFLURAMINE; D-FENFLURAMINE; WEIGHT CONTROL; URINE; DEXFENFLURAMINE; DERIVATIZATION; DERIVATIVES; PHENTERMINE; METABOLISM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Nakashima, K Nagasaki Univ, Grad Sch Pharmaceut Sci, Dept Analyt Res Pharmacoinformat, 1-14 Bunkyo Machi, Nagasaki 852, Japan Nagasaki Univ 1-14 Bunkyo Machi Nagasaki Japan 852 52, Japan
Citazione:
A. Kaddoumi et al., "High performance liquid chromatography with UV detection for the simultaneous determination of sympathomimetic amines using 4-(4,5-diphenyl-1H-imidazole-2-yl)benzoyl chloride as a label", BIOMED CHRO, 15(6), 2001, pp. 379-388

Abstract

A high performance liquid chromatographic method has been developed for the simultaneous determination of () fenfluramine (Fen) and phentermine (Phen) in addition to three other sympathomimetic amines-ephedrine (E), norephedrine (NE) and 2-phenylethylamine (2-PEA), using cyclohexylamine (CX) as an internal standard in plasma. The compounds were derivatized with 4-(4,5-diphenyl-1H-imidazole-2-yl)benzoyl chloride (DIB-Cl) to give the DIB-derivatives. The derivatives were then separated using an isocratic HPLC system withUV detection. The limits of detection for Fen, Phen, E, NE and 2-PEA in plasma ranged from 0.32 to 22.9 pmol on column at a signal-to-noise ratio of 3. The recoveries following alkaline extraction from plasma samples of known concentrations were found to be more than 94% for the studied compounds. This method might be useful for the screening of the studied sympathomimetic amines in human plasma samples in forensic as well as toxicological studies. Furthermore, the developed method was modified for the simultaneous determination of Fen and Phen in human and rat plasma using fluoxetine as an internal standard. The methods are reproducible and precise. Finally, the two drugs were administered intraperitonealy to rats in combination, and their plasma levels over the investigated time course were successfully determined. Copyright (C) 2001 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 02:48:37