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Titolo:
The stiffness of bone marrow cell-knit composites is increased during mechanical load
Autore:
Bruinink, A; Siragusano, D; Ettel, G; Brandsberg, T; Brandsberg, F; Petitmermet, M; Muller, B; Mayer, J; Wintermantel, E;
Indirizzi:
ETH Zurich, Swiss Fed Inst Technol, Dept Mat Biocompatible Mat Sci & Engn,CH-8952 Schlieren, Switzerland ETH Zurich Schlieren Switzerland CH-8952 ,CH-8952 Schlieren, Switzerland
Titolo Testata:
BIOMATERIALS
fascicolo: 23, volume: 22, anno: 2001,
pagine: 3169 - 3178
SICI:
0142-9612(200112)22:23<3169:TSOBMC>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VITRO; SIGNAL-TRANSDUCTION; HUMAN OSTEOBLASTS; TISSUE-CULTURE; STROMAL CELLS; STRAIN; MECHANOTRANSDUCTION; PROLIFERATION; FIBROBLASTS; RESPONSES;
Keywords:
mechanical load; culture; bone marrow; knit; stiffness; strain;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Bruinink, A EMPA St Gallen, Lerchenfeldstr 5, CH-9014 St Gallen, Switzerland EMPA St Gallen Lerchenfeldstr 5 St Gallen Switzerland CH-9014
Citazione:
A. Bruinink et al., "The stiffness of bone marrow cell-knit composites is increased during mechanical load", BIOMATERIAL, 22(23), 2001, pp. 3169-3178

Abstract

A novel device for mechanical stimulation of primary adult rat bone marrowcells cultured on three-dimensional knitted textiles has been prototyped. A method has been developed ensuring a well-defined, high-density, and reproducible cell seeding on the knitted fabric. After culturing for 18-52 daysthe cell-knit composites were subjected to uniaxial 2% stretching and relaxation. The frequency was altered between 0.1 Hz (196 min, loading phase) and 0.01 Hz (360 min, resting phase). Identically treated knits without cells exhibited a slight stiffness reduction, whereas the stiffness of knits with cells increased from cycle to cycle. The stiffness increase was found todepend on the duration of the culture period before mechanical loading. Our data suggest that the extracellular matrix deposited by the cells on the knit and intact microtubuli of living cells cause the observed stiffness increase. In comparison to the unstrained static cell-knit composites cell proliferation and bone cell differentiation were reduced by the mechanical load. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 10:11:34