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Titolo:
Application of the gene search system to screen for longevity genes in Drosophila
Autore:
Seong, KH; Ogashiwa, T; Matsuo, T; Fuyama, Y; Aigaki, T;
Indirizzi:
Tokyo Metropolitan Univ, Dept Biol Sci, Hachioji, Tokyo 1920397, Japan Tokyo Metropolitan Univ Hachioji Tokyo Japan 1920397 Tokyo 1920397, Japan Japan Sci & Technol Corp, Precursory Res Embryon Sci & Technol, Suita, Osaka 5650082, Japan Japan Sci & Technol Corp Suita Osaka Japan 5650082 , Osaka 5650082, Japan
Titolo Testata:
BIOGERONTOLOGY
fascicolo: 3, volume: 2, anno: 2001,
pagine: 209 - 217
SICI:
1389-5729(2001)2:3<209:AOTGSS>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIFE-SPAN; CAENORHABDITIS-ELEGANS; SUPEROXIDE-DISMUTASE; OXIDATIVE STRESS; MELANOGASTER; EXPRESSION; OVEREXPRESSION; RESISTANCE; RNA; THIOREDOXIN;
Keywords:
Drosophila melanogaster; gain-of-function screen; gene search system; longevity; paraquat resistance;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Aigaki, T Tokyo Metropolitan Univ, Dept Biol Sci, 1-1 Minami Osawa, Hachioji, Tokyo 1920397, Japan Tokyo Metropolitan Univ 1-1 Minami Osawa Hachioji Tokyo Japan 1920397
Citazione:
K.H. Seong et al., "Application of the gene search system to screen for longevity genes in Drosophila", BIOGERONTOL, 2(3), 2001, pp. 209-217

Abstract

We have developed a strategy using Drosophilo as a model system to identify genes that are crucial for extension of longevity. A collection of transgenic lines with a P-element based gene search (GS) vector containing UAS (Upstream Activating Sequence) was screened for longevity in combination withan hsp70 promoter-driven GAL4 transgene. Misexpression of the vector-flanking sequence was induced throughout the adult stage to assess its effects on the aging process rather than development. We showed that the longevity was greatly affected by GS inserts, and it was positively correlated with paraquat resistance. Of 646 GS inserts, we selected 23 inserts with relatively longer longevity for further molecular analysis. All of the misexpressed sequences matched either known genes or ESTs (Expressed Sequence Tags). Among 13 genes whose functions are already known or suggested, six were related to stress resistance or redox balance (DmGST2, hsp26, nla, and Drosophilahomologs of mammalian TR GILT and POSH), suggesting the importance of stress resistance for the extension of longevity. This is the first demonstration that a systematic gain-of-function screen could efficiently detect longevity genes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 20:38:30