Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Role of scavenger receptors SR-BI and CD36 in selective sterol uptake in the small intestine
Autore:
Werder, M; Han, CH; Wehrli, E; Bimmler, D; Schulthess, G; Hauser, H;
Indirizzi:
ETH Ctr, Inst Biochem, CH-8092 Zurich, Switzerland ETH Ctr Zurich Switzerland CH-8092 Biochem, CH-8092 Zurich, Switzerland Univ Zurich Hosp, Dept Visceral & Transplantat Surg, CH-8091 Zurich, Switzerland Univ Zurich Hosp Zurich Switzerland CH-8091 CH-8091 Zurich, Switzerland Univ Zurich Hosp, Med Policlin, Dept Internal Med, CH-8091 Zurich, Switzerland Univ Zurich Hosp Zurich Switzerland CH-8091 CH-8091 Zurich, Switzerland
Titolo Testata:
BIOCHEMISTRY
fascicolo: 38, volume: 40, anno: 2001,
pagine: 11643 - 11650
SICI:
0006-2960(20010925)40:38<11643:ROSRSA>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
BRUSH-BORDER MEMBRANE; HIGH-DENSITY-LIPOPROTEIN; CELLULAR CHOLESTEROL EFFLUX; UNILAMELLAR VESICLES; ABSORPTION; EXCHANGE; MODELS; PHOSPHATIDYLCHOLINE; APOLIPOPROTEINS; PROTEINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Hauser, H ETH Ctr, Inst Biochem, Univ Str 16, CH-8092 Zurich, Switzerland ETH Ctr Univ Str 16 Zurich Switzerland CH-8092 ich, Switzerland
Citazione:
M. Werder et al., "Role of scavenger receptors SR-BI and CD36 in selective sterol uptake in the small intestine", BIOCHEM, 40(38), 2001, pp. 11643-11650

Abstract

The serum lipoprotein high-density lipoprotein (HDL), which is a ligand ofscavenger receptors such as scavenger receptor class B type I (SR-BI) and cluster determinant 36 (CD36), can act as a donor particle for intestinal lipid uptake into the brush border membrane (BBM). Both cholesterol and phospholipids are taken up by the plasma membrane of BBM vesicles (BBMV) and Caco-2 cells in a facilitated (protein-mediated) process. The protein-mediated transfer of cholesterol from reconstituted HDL to BBMV depends on the lipid composition of the HDL. In the presence of sphingomyelin, the transfer of cholesterol is slowed by a factor of about 3 probably due to complex formation between cholesterol and the sphingolipid. It is shown that the mechanism of lipid transfer from reconstituted HDL to either BBMV or Caco-2 cellsas the acceptor is consistent with selective lipid uptake: the lipid donordocks at the membrane-resident scavenger receptors which mediate the transfer of lipids between donor and acceptor. Selective lipid uptake implies that lipid, but no apoprotein is transferred from the donor to the BBM, thus excluding endocytotic processes. The two BBM models used here clearly indicate that fusion of donor particles with the BBM can be ruled out as a majormechanism contributing to intestinal lipid uptake. Here we demonstrate that CD36, another member of the family of scavenger receptors, is present in rabbit and human BBM vesicles. This receptor mediates the uptake of free cholesterol, but not of esterified cholesterol, the uptake of which is mediated exclusively by SR-BI. More than one scavenger receptor appears to be involved in the uptake of free cholesterol with SR-BI contributing about 25% and CD36 about 35%. There is another yet unidentified protein accounting forthe remaining 30 to 40%.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 18:38:58