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Titolo:
Four Trypanosoma brucei fatty acyl-CoA synthetases: fatty acid specificityof the recombinant proteins
Autore:
Jiang, DW; Englund, PT;
Indirizzi:
Johns Hopkins Med Sch, Dept Biol Chem, Baltimore, MD 21205 USA Johns Hopkins Med Sch Baltimore MD USA 21205 hem, Baltimore, MD 21205 USA
Titolo Testata:
BIOCHEMICAL JOURNAL
, volume: 358, anno: 2001,
parte:, 3
pagine: 757 - 761
SICI:
0264-6021(20010915)358:<757:FTBFAS>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
VARIANT SURFACE GLYCOPROTEIN; MYRISTATE; SEQUENCE; ANCHORS; GENES;
Keywords:
ACS signature motif; enzyme-coupled assay; fatty acid chain length; glycosyl phosphatidylinositol myristoylation; recombinant acyl-CoA synthetases (ACS);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Englund, PT Johns Hopkins Med Sch, Dept Biol Chem, 725 N Wolfe St, Baltimore, MD 21205USA Johns Hopkins Med Sch 725 N Wolfe St Baltimore MD USA 21205SA
Citazione:
D.W. Jiang e P.T. Englund, "Four Trypanosoma brucei fatty acyl-CoA synthetases: fatty acid specificityof the recombinant proteins", BIOCHEM J, 358, 2001, pp. 757-761

Abstract

As part of our investigation of fatty acid metabolism in Trypanosoma brucei, we have expressed four acyl-CoA synthetase (TbACS) genes in Esherichia coli. The recombinant proteins, with His-tags on their C-termini, were purified to near homogeneity using nickel-chelate affinity chromatography. Although these. enzymes are highly homologous, they have distinct specificities for fatty acid chain length. TbACS1 prefers saturated fatty acids in the range C-11:0 to C-14:0 and TbACS2 prefers shorter fatty acids, mainly C-10:0. TbACS3 and 4, which have 95% sequence identity, have similar specificities, favouring fatty acids between C-14:0 and C-17:0. In addition, TbACS1, 3 and 4 function well with a variety of unsaturated fatty acids.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 00:24:32