Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The inhibitory effects of camptothecin, a topoisomerase I inhibitor, on collagen synthesis in fibroblasts from patients with systemic sclerosis
Autore:
Czuwara-Ladykowska, J; Makiela, B; Smith, EA; Trojanowska, M; Rudnicka, L;
Indirizzi:
Med Univ S Carolina, Dept Med, Div Rheumatol & Immunol, Charleston, SC 29425 USA Med Univ S Carolina Charleston SC USA 29425 nol, Charleston, SC 29425 USA Warsaw Acad Med & Hosp, Dept Dermatol, Warsaw, Poland Warsaw Acad Med & Hosp Warsaw Poland osp, Dept Dermatol, Warsaw, Poland
Titolo Testata:
ARTHRITIS RESEARCH
fascicolo: 5, volume: 3, anno: 2001,
pagine: 311 - 318
SICI:
1465-9913(2001)3:5<311:TIEOCA>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR-BETA; NF-KAPPA-B; GENE-EXPRESSION; MESSENGER-RNA; TRANSCRIPTION; SCLERODERMA; AUTOANTIBODIES; CHEMOTHERAPY; ACTIVATION; COMPLEX;
Keywords:
camptothecin; collagen; fibroblast; systemic sclerosis; topoisomerase I;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Czuwara-Ladykowska, J Med Univ S Carolina, Dept Med, Div Rheumatol & Immunol, 96 Jonathan Lucas St, Charleston, SC 29425 USA Med Univ S Carolina 96 Jonathan Lucas St Charleston SC USA 29425
Citazione:
J. Czuwara-Ladykowska et al., "The inhibitory effects of camptothecin, a topoisomerase I inhibitor, on collagen synthesis in fibroblasts from patients with systemic sclerosis", ARTHRITIS R, 3(5), 2001, pp. 311-318

Abstract

The main manifestation of systemic sclerosis (SSc) is the overproduction of extracellular matrix, predominantly type I collagen. This study was undertaken to evaluate the effects of noncytotoxic doses of the topoisomerase I inhibitor camptothecin (CPT) on collagen production in the activated dermalfibroblasts from patients with SSc and healthy donors. The fibroblasts were cultured in the presence or absence of CPT. Production of collagenous proteins by fibroblasts was determined in cell and matrix layers by ELISA and in conditioned media by [H-3]proline incorporation, gel electrophoresis, and autoradiography. Expression of alpha2(I) collagen (COL1A2) mRNA was measured by northern blot, and the activity of COL1A2 promoter was determined bya chloramphenicol acetyltransferase, assay. CPT (10(-7) M) decreased the deposition of type I collagen by 68%, of type III by 38%, and of type VI by 21% in SSc fibroblasts and to a lesser degree in healthy controls. Similarly, CPT (10(-8) M to 10(-6) M) significantly inhibited secretion of newly synthesized collagenous proteins into conditioned media by 50%. CPT (10(-8) Mto 10(-6) M) caused a significant dose-dependent inhibition of COL1A2 mRNAlevels and COL1A2 promoter activity, both by as much as 60%. The inhibitory effect of CPT on collagen production by fibroblasts from patients with SSc suggests that topoisomerase I inhibitors may be effective in limiting fibrosis in such patients.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 07:59:10