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Titolo:
Biomonitoring of continuous microbial community adaptation towards more efficient phenol-degradation in a fed-batch bioreactor
Autore:
Guieysse, B; Wikstrom, P; Forsman, M; Mattiasson, B;
Indirizzi:
Lund Univ, Dept Biotechnol, Ctr Chem & Chem Engn, S-22100 Lund, Sweden Lund Univ Lund Sweden S-22100 Ctr Chem & Chem Engn, S-22100 Lund, Sweden Swedish Def Res Agcy, Dept NBC Anal, S-90182 Umea, Sweden Swedish Def Res Agcy Umea Sweden S-90182 NBC Anal, S-90182 Umea, Sweden Swedish Def Res Agcy, Dept NBC Anal Microbiol, S-90182 Umea, Sweden Swedish Def Res Agcy Umea Sweden S-90182 Microbiol, S-90182 Umea, Sweden
Titolo Testata:
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
fascicolo: 5-6, volume: 56, anno: 2001,
pagine: 780 - 787
SICI:
0175-7598(200109)56:5-6<780:BOCMCA>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMPLIFIED POLYMORPHIC DNA; POLYCYCLIC AROMATIC-HYDROCARBONS; 16S RIBOSOMAL-RNA; WASTE-WATER; DENITRIFYING BACTERIA; ANAEROBIC DEGRADATION; BIODEGRADATION; ACCLIMATION; GENES; RAPD;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Mattiasson, B Lund Univ, Dept Biotechnol, Ctr Chem & Chem Engn, POB 124, S-22100 Lund, Sweden Lund Univ POB 124 Lund Sweden S-22100 S-22100 Lund, Sweden
Citazione:
B. Guieysse et al., "Biomonitoring of continuous microbial community adaptation towards more efficient phenol-degradation in a fed-batch bioreactor", APPL MICR B, 56(5-6), 2001, pp. 780-787

Abstract

The anaerobic degradation of phenol was studied in a fed-batch culture. Nitrate was added as electron acceptor and phenol was provided three times, to a final concentration of 200 mg/l. Randomly amplified polymorphic DNA (RAPD) and terminal fraction fragment length polymorphism (T-RFLP) were used and compared in order to monitor the microbial succession in the reactor. Phenol degradation started after an initial lag phase of 14 days and was thencompleted within a few days. In addition. the duration of the lag phase was shortened and the degradation rate was increased after each phenol amendment. Nitrate reduction correlated with microbial growth and phenol depletion, confirming that the degradation was carried out anaerobically. Results from the DNA analysis showed that the structure of the microbial community changed after each phenol amendment. This study confirms the potential for anaerobic degradation of environmental pollutants and also confirms that microbial acclimation towards faster degradation rates occurred upon repeated substrate amendments. Furthermore. both of the DNA-based techniques described the phenol degradation-linked community shifts with similar general results. RAPD is a faster, simpler technique that gives a higher resolution andconsequently reflects the shifts in the microbial community structure better. whereas T-RFLP is more suitable for phylogenetic studies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 13:47:04