Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Telomere length and telomerase activity in canine mammary gland tumors
Autore:
Yazawa, M; Okuda, M; Setoguchi, A; Iwabuchi, S; Nishimura, R; Sasaki, N; Masuda, K; Ohno, K; Tsujimoto, H;
Indirizzi:
Univ Tokyo, Grad Sch Agr & Life Sci, Dept Vet Internal Med, Tokyo 1138657,Japan Univ Tokyo Tokyo Japan 1138657 ept Vet Internal Med, Tokyo 1138657,Japan Univ Tokyo, Grad Sch Agr & Life Sci, Dept Vet Surg, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 Sci, Dept Vet Surg, Tokyo 1138657, Japan Yamaguchi Univ, Fac Agr, Lab Vet Internal Med, Yamaguchi 7538515, Japan Yamaguchi Univ Yamaguchi Japan 7538515 nal Med, Yamaguchi 7538515, Japan Nippon Zenyaku Kogyo, Cent Res Labs, Koriyama, Fukushima 9630196, Japan Nippon Zenyaku Kogyo Koriyama Fukushima Japan 9630196 hima 9630196, Japan
Titolo Testata:
AMERICAN JOURNAL OF VETERINARY RESEARCH
fascicolo: 10, volume: 62, anno: 2001,
pagine: 1539 - 1543
SICI:
0002-9645(200110)62:10<1539:TLATAI>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN FIBROBLASTS; HUMAN-CHROMOSOMES; IMMORTAL CELLS; FISSION YEAST; END; INHIBITION; MECHANISM; (TTAGGG)N; PROTEINS; SEQUENCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Yazawa, M Univ Tokyo, Grad Sch Agr & Life Sci, Dept Vet Internal Med, Tokyo 1138657,Japan Univ Tokyo Tokyo Japan 1138657 ternal Med, Tokyo 1138657,Japan
Citazione:
M. Yazawa et al., "Telomere length and telomerase activity in canine mammary gland tumors", AM J VET RE, 62(10), 2001, pp. 1539-1543

Abstract

Objective-To measure telomere length and telomerase activity in naturally occurring canine mammary gland tumors. Sample Population-27 mammary gland tumor specimens obtained during resection or necropsy and 12 mammary gland tissue specimens obtained from healthy (control) dogs. Procedure-Telomere length in tissue specimens was measured by use of restriction endonuclease digestion and Southern blot analysis. Telomerase activity was measured by use of a telomeric. repeat amplification protocol assay. Results-Telomere length in mammary gland tumors ranged from 11.0 to 21.6 kilobase pairs (kbp; mean SEM, 14.5 +/- 0.5 kbp) but did not differ among tumor types. Telomeres in mammary gland tumors were slightly shorter than in normal tissue specimens, but telomere length could not be directly comparedbetween groups, because mean age of dogs was significantly different between groups. Age was negatively correlated with telomere length In control dogs but was not significantly correlated with length in affected dogs. Telomerase activity was detected In 26 of 27 mammary gland tumors and in 4 of 12normal tissue specimens. However, telomerase activity and telomere length were not correlated in tumor specimens. Conclusions and Clinical Relevance-Telomere length is maintained In caninemammary gland tumors regardless of the age of the affected dog. Measurement of telomere length may be a useful tool for monitoring the in vivo effects of telomerase inhibitors in dogs with tumors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 17:42:49