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Titolo:
Altered K+ channel gene expression in diabetic rat ventricle: isoform switching between Kv4.2 and Kv1.4
Autore:
Nishiyama, A; Ishii, DN; Backx, PH; Pulford, BE; Birks, BR; Tamkun, MM;
Indirizzi:
Colorado State Univ, Dept Physiol, Ft Collins, CO 80523 USA Colorado StateUniv Ft Collins CO USA 80523 iol, Ft Collins, CO 80523 USA Colorado State Univ, Dept Biochem & Mol Biol, Ft Collins, CO 80523 USA Colorado State Univ Ft Collins CO USA 80523 iol, Ft Collins, CO 80523 USA Univ Toronto, Dept Med, Cardiovasc Res Ctr, Toronto, ON M5G 2C4, Canada Univ Toronto Toronto ON Canada M5G 2C4 s Ctr, Toronto, ON M5G 2C4, Canada Univ Toronto, Toronto Hosp, Toronto, ON M5G 2C4, Canada Univ Toronto Toronto ON Canada M5G 2C4 Hosp, Toronto, ON M5G 2C4, Canada
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
fascicolo: 4, volume: 281, anno: 2001,
pagine: H1800 - H1807
SICI:
0363-6135(200110)281:4<H1800:AKCGEI>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR-I; TRANSIENT OUTWARD CURRENT; MESSENGER-RNA EXPRESSION; POTASSIUM CURRENTS; CARDIOVASCULAR-SYSTEM; AUTONOMIC NEUROPATHY; ALPHA-SUBUNITS; THYROID STATUS; INSULIN; MYOCYTES;
Keywords:
potassium channel expression; diabetes; insulin-like growth factor; hypertrophy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Tamkun, MM Colorado State Univ, Dept Physiol, Ft Collins, CO 80523 USA Colorado State Univ Ft Collins CO USA 80523 lins, CO 80523 USA
Citazione:
A. Nishiyama et al., "Altered K+ channel gene expression in diabetic rat ventricle: isoform switching between Kv4.2 and Kv1.4", AM J P-HEAR, 281(4), 2001, pp. H1800-H1807

Abstract

Expression of voltage-gated K+ channels encoding the K+ independent transient outward current in the streptozocin-induced diabetic (DM) rat ventriclewas studied to determine the basis for slowed cardiac repolarization in diabetes mellitus. Although hypertrophy was not detected in diabetic rats at 12 wk after streptozocin treatment ventricular Kv4.2 mRNA levels decreased 41% relative to nondiabetic controls. Kv1.4 mRNA levels increased 179% relative to controls, whereas Kv4.3 mRNA levels were unaffected. Immunohistochemistry and Western blot analysis of the diabetic heart showed that the density of the Kv4.2 protein decreased, whereas Kv1.4 protein increased. Thus isoform switching from Kv4.2 to Kv1.4 is most likely the mechanism underlying the slower kinetics of transient outward K+ current observed in the diabetic ventricle. Brain Kv1.4, Kv4.2, or Kv4.3 mRNA levels were unaffected by diabetes. Myosin heavy chain (MHC) gene expression was altered with a 32% decrease in alpha -MHC mRNA and a 259% increase in beta -MHC mRNA levels in diabetic ventricle. Low-dose insulin-like growth factor-II (IGF-II) treatment during the last 6 of the 12 wk of diabetes (DM + IGF) protected against these changes in MHC mRNAs despite continued hyperglycemia and body weight loss. IGF-II treatment did not change K+ channel mRNA levels in DM or control rat ventricles. Thus IGF treatment may prevent some, but not all, biochemical abnormalities in the diabetic heart.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 03:54:04