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Titolo:
Ischemic and anesthetic preconditioning reduces cytosolic [Ca2+] and improves Ca2+ responses in intact hearts
Autore:
An, JZ; Varadarajan, SG; Novalija, E; Stowe, DF;
Indirizzi:
Med Coll Wisconsin, Milwaukee Reg Med Ctr, Dept Anesthesiol, Anesthesiol Res Labs, Milwaukee, WI 53226 USA Med Coll Wisconsin Milwaukee WI USA 53226s Labs, Milwaukee, WI 53226 USA Med Coll Wisconsin, Dept Physiol, Milwaukee, WI 53226 USA Med Coll Wisconsin Milwaukee WI USA 53226 hysiol, Milwaukee, WI 53226 USA Med Coll Wisconsin, Cardiovasc Res Ctr, Milwaukee, WI 53226 USA Med Coll Wisconsin Milwaukee WI USA 53226 es Ctr, Milwaukee, WI 53226 USA Vet Affairs Med Ctr, Res Serv, Milwaukee, WI 53295 USA Vet Affairs Med Ctr Milwaukee WI USA 53295 Serv, Milwaukee, WI 53295 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
fascicolo: 4, volume: 281, anno: 2001,
pagine: H1508 - H1523
SICI:
0363-6135(200110)281:4<H1508:IAAPRC>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; MYOCARDIAL INFARCT SIZE; SENSITIVE POTASSIUM CHANNELS; PIG ISOLATED HEARTS; K-ATP CHANNEL; SARCOPLASMIC-RETICULUM; CONTRACTILE FUNCTION; STUNNED MYOCARDIUM; CORONARY-OCCLUSION; CALCIUM OVERLOAD;
Keywords:
experimental; cellular; pathophysiology; acidosis; contractile function;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Stowe, DF Med Coll Wisconsin, Milwaukee Reg Med Ctr, Dept Anesthesiol, Anesthesiol Res Labs, Box M4280,8701 Watertown Plank Rd, Milwaukee, WI 53226 USA Med Coll Wisconsin Box M4280,8701 Watertown Plank Rd Milwaukee WI USA 53226
Citazione:
J.Z. An et al., "Ischemic and anesthetic preconditioning reduces cytosolic [Ca2+] and improves Ca2+ responses in intact hearts", AM J P-HEAR, 281(4), 2001, pp. H1508-H1523

Abstract

Ca+ loading during reperfusion after myocardial ischemia is linked to reduced cardiac function. Like ischemic preconditioning (IPC), a volatile anesthetic given briefly before ischemia can reduce reperfusion injury. We determined whether IPC and sevoflurane preconditioning (SPC) before ischemia equivalently improve mechanical and metabolic function, reduce cytosolic Ca2+ loading, and improve myocardial Ca2+ responsiveness. Four groups of guinea pig isolated hearts were perfused: no ischemia, no treatment before 30-min global ischemia and 60-min reperfusion (control), IPC (two 2-min occlusions) before ischemia, and SPC (3.5 vol%, two 2-min exposures) before ischemia. Intracellular Ca2+ concentration ([Ca2+](i)) was measured at the left ventricular (LV) free wall with the fluorescent probe indo 1. Ca2+ responsiveness was assessed by changing extracellular [Ca2+]. In control hearts, initial reperfusion increased diastolic [Ca2+] and diastolic LV pressure (LVP), and the maximal and minimal derivatives of LVP (dLVP/dt(max) and dLVP/dt(min), respectively), O-2 consumption, and cardiac efficiency (CE). Throughout reperfusion, IPC and SPC similarly reduced ischemic contracture, ventricular fibrillation, and enzyme release, attenuated rises in systolic and diastolic [Ca2+], improved contractile and relaxation indexes, O-2 consumption, and CE, and reduced infarct size. Diastolic [Ca2+] at 50% dLVP/dt(min) was right shifted by 32-53 +/- nM after 30-min reperfusion for all groups. Phasic [Ca2+] at 50% dLVP/dt(max) was not altered in control but was left shifted by -235 +/- 40 nM [Ca2+] after IPC and by -135 +/- 20 nM [Ca2+] after SPC. Both SPC and IPC similarly reduce Ca2+ loading, while augmenting contractile responsiveness to Ca2+, improving postischemia cardiac function and attenuating permanent damage.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 00:12:45