Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Identification of pestiviruses contaminating cell lines and fetal calf sera
Autore:
Vilcek, S;
Indirizzi:
Univ Vet Med, Kosice 04181, Slovakia Univ Vet Med Kosice Slovakia 04181Univ Vet Med, Kosice 04181, Slovakia
Titolo Testata:
ACTA VIROLOGICA
fascicolo: 2, volume: 45, anno: 2001,
pagine: 81 - 86
SICI:
0001-723X(200104)45:2<81:IOPCCL>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
VIRAL DIARRHEA VIRUS; BORDER DISEASE VIRUS; CULTURES; VACCINES; RNA; GENOTYPES; ANTIBODY; PIGS; PCR;
Keywords:
pestivirus; cell line; fetal calf serum; RT-PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Vilcek, S Univ Vet Med, Komenskeho 73, Kosice 04181, Slovakia Univ Vet MedKomenskeho 73 Kosice Slovakia 04181 4181, Slovakia
Citazione:
S. Vilcek, "Identification of pestiviruses contaminating cell lines and fetal calf sera", ACT VIROLOG, 45(2), 2001, pp. 81-86

Abstract

Three pestiviruses in fetal calf serum (FCS), a pestivirus in porcine ST cell line, and two pestiviruses in two of five bovine cell lines were detected by RT-PCR method employing panpestivirus primers selected from the 5'-non-coding region (5'-NCR). The 288 bp products were sequenced in both directions. To identify these pestiviruses, their nucleotide sequences and those of reference pestiviruses were used for construction of a dendrogram. Threeviruses present in FCS and a virus present in the bovine RP-15 cell line were identified as bovine viral diarrhea virus 1 (BVDV-1). Bovine viral diarrhea virus 2 (BVDV-2) was identified in a batch of the bovine MDBK cell line. A pestivirus contaminating the porcine ST cell line was identified as a Border disease virus (BDV).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/08/20 alle ore 20:58:22