Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Immortalized goat milk epithelial cell lines replicate CAEV at high level
Autore:
Mselli-Lakhal, L; Guiguen, F; Fornazero, C; Favier, C; Durand, J; Grezel, D; Moussa, A; Mornex, JF; Chebloune, Y;
Indirizzi:
Ecole Natl Lyon, UCBL 754, ENVL, UMR INRA, F-69280 Marcy Letoile, France Ecole Natl Lyon Marcy Letoile France F-69280 69280 Marcy Letoile, France AFSSA, Lab Etud & Rech Pathol Bovine, F-69342 Lyon 07, France AFSSA LyonFrance 07 Etud & Rech Pathol Bovine, F-69342 Lyon 07, France
Titolo Testata:
VETERINARY RESEARCH
fascicolo: 5, volume: 32, anno: 2001,
pagine: 429 - 440
SICI:
0928-4249(200109/10)32:5<429:IGMECL>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; ARTHRITIS-ENCEPHALITIS-VIRUS; RESPIRATORY SYNCYTIAL VIRUS; CAPRINE ARTHRITIS; GENE-EXPRESSION; HIV-INFECTION; A INFECTION; IN-VITRO; TYPE-1; ENTEROPATHY;
Keywords:
CAEV; replication; epithelial cell line;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Chebloune, Y Ecole Natl Lyon, UCBL 754, ENVL, UMR INRA, BP 83, F-69280 Marcy Letoile, France Ecole Natl Lyon BP 83 Marcy Letoile France F-69280 e, France
Citazione:
L. Mselli-Lakhal et al., "Immortalized goat milk epithelial cell lines replicate CAEV at high level", VET RES, 32(5), 2001, pp. 429-440

Abstract

Primary milk epithelial cells were isolated from CAEV-uninfected goats andthree cell lines designated TIGMEC-1, TIGMEC-2 and TIGMEC-3 were established. The three cell lines retained their morphological characteristics of epithelial cells and expressed specific epithelial cytokeratin marker as wellas the immortalizing SV40 large T antigen. The kinetics of growth of TIGMEC1, TIGMEC2 and TIGMEC3 cell lines showed a doubling time of 24-48 hours while the parental cell lines became senescent after the passage 6 in cell culture. Like the parental primary cells, the three cell lines were found to be highly sensitive to CAEV-pBSCA, an infectious molecular clone of CAEV-COstrain, and to a French isolate CAEV-3112. TIGMEC cell lines infected withCAEV-pBSCA became chronically infected producing high virus titers in absence of cytopathic effects. These cell lines may be useful for study of the possible physiological alterations in mammary epithelial cells infected with small ruminant lentiviruses and their consequences on milk quality. On another hand, these cell lines can be used to study their role in virus transmission and pathogenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 12:46:25