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Titolo:
Induction of cross clade reactive specific antibodies in mice by conjugates of HGP-30 (peptide analog of HIV-1(SF2) p17) and peptide segments of human beta-2-microglobulin or MHC II beta chain
Autore:
Zimmerman, DH; Lloyd, JP; Heisey, D; Winship, MD; Siwek, M; Talor, E; Sarin, PS;
Indirizzi:
CEL SCI Corp, Vienna, VA 22182 USA CEL SCI Corp Vienna VA USA 22182CEL SCI Corp, Vienna, VA 22182 USA
Titolo Testata:
VACCINE
fascicolo: 32, volume: 19, anno: 2001,
pagine: 4750 - 4759
SICI:
0264-410X(20010914)19:32<4750:IOCCRS>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; HERPES-SIMPLEX VIRUS; CELL-MEDIATED-IMMUNITY; T-LYMPHOCYTE CLONES; SYNTHETIC PEPTIDES; HIV-INFECTION; TYPE-1 INFECTION; HELPER EPITOPES; 38-KDA PROTEIN; BINDING-SITE;
Keywords:
HIV-1; HIV-1 antibodies; p17; HGP-30; vaccine; beta-2-microglobulin; MHC II beta chain; peptide conjugate;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
65
Recensione:
Indirizzi per estratti:
Indirizzo: Zimmerman, DH CEL SCI Corp, 8229 Boone Blvd,Suite 802, Vienna, VA 22182 USA CEL SCI Corp 8229 Boone Blvd,Suite 802 Vienna VA USA 22182 A
Citazione:
D.H. Zimmerman et al., "Induction of cross clade reactive specific antibodies in mice by conjugates of HGP-30 (peptide analog of HIV-1(SF2) p17) and peptide segments of human beta-2-microglobulin or MHC II beta chain", VACCINE, 19(32), 2001, pp. 4750-4759

Abstract

HGP-30, a 30 amino acid synthetic peptide homologous to a conserved regionof HIV-1(SF2) p17 (aa86-115), has previously been shown to elicit both cellular and Immoral immune responses when conjugated to KLH and adsorbed to alum. However, the free HGP-30 peptide is not immunogenic in animals. In order to improve the immunogenicity of HGP-30, peptide conjugates consisting of a modified HGP-30 sequence (m-HGP-30/aa82-111) and a peptide segment, residues 38-50, of the MHC I accessory molecule, human beta -2-microglobulin (beta -2-M), referred to as Peptide J, or a peptide from the MHC II beta chain (peptide G) were evaluated in mice. The effects of carriers and adjuvants on serum antibody titers, specificities to various HIV-1 clade peptides similar to HGP-30 and isotype patterns were examined. Peptides J or especially G conjugated to modified-HGP-30 (LEAPS 102 and LEAPS 101, respectively) generated comparable or better immune responses to modified HGP-30 than KLHconjugates as judged by the induction of. (1) similar antibody titers; (2)broader HIV clade antigen binding; and (3) antibody isotype response patterns indicative of a TH1 pathway (i.e. increased amounts of IgG2a and IgG2b antibodies). The ISA 51 and MPL (R) -SE adjuvants induced higher antibody responses than alum, with the ISA 51 being more potent. Immune responses to LEAPS 102, as compared to LEAPS 101, were weaker and slower to develop as determined by antibody titers and cross clade reactivity of the antibodies induced. Compared to KLH conjugates which induced significant anti-KLH antibody titers, minimal antibody responses were observed to peptide G, the moreimmunogenic conjugate, and peptide J. These results suggest that modified HGP-30 L.E.A.P.S. constructs may be useful as HIV vaccine candidates for preferential induction of TH1 directed cell mediated immune responses. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 19:33:12