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Titolo:
A prime-boost immunisation regimen using DNA followed by recombinant modified vaccinia virus Ankara induces strong cellular immune responses against the Plasmodium falciparum TRAP antigen in chimpanzees
Autore:
Schneider, J; Langermans, JAM; Gilbert, SC; Blanchard, TJ; Twigg, S; Naitza, S; Hannan, CM; Aidoo, M; Crisanti, A; Robson, KJ; Smith, GL; Hill, AVS; Thomas, AW;
Indirizzi:
Univ Oxford, John Radcliffe Hosp, Nuffield Dept Med, Inst Mol Med,Mol Immunol Grp, Oxford OX3 9DU, England Univ Oxford Oxford England OX3 9DU Immunol Grp, Oxford OX3 9DU, England BPRC, Dept Parasitol, NL-2280 GH Rijswijk, Netherlands BPRC Rijswijk Netherlands NL-2280 GH l, NL-2280 GH Rijswijk, Netherlands Univ Oxford, Wellcome Trust Ctr Human Genet, Oxford OX3 7BN, England Univ Oxford Oxford England OX3 7BN Human Genet, Oxford OX3 7BN, England Univ Oxford, Sir William Dunn Sch Pathol, Oxford OX1 3RE, England Univ Oxford Oxford England OX1 3RE n Sch Pathol, Oxford OX1 3RE, England Univ London Imperial Coll Sci Technol & Med, Dept Pure & Appl Biol, Mol & Cellular Parasitol Grp, London SW7 2BB, England Univ London Imperial Coll Sci Technol & Med London England SW7 2BB gland Univ Oxford, Inst Mol Med, MRC, Mol Haematol Unit, Oxford OX3 9DS, EnglandUniv Oxford Oxford England OX3 9DS aematol Unit, Oxford OX3 9DS, England
Titolo Testata:
VACCINE
fascicolo: 32, volume: 19, anno: 2001,
pagine: 4595 - 4602
SICI:
0264-410X(20010914)19:32<4595:APIRUD>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
T-CELLS; HEPATITIS-B; CIRCUMSPOROZOITE PROTEIN; PROTECTIVE EFFICACY; NONHUMAN-PRIMATES; MALARIA VACCINE; HIV-1 CHALLENGE; IMMUNIZATION; IMMUNOGENICITY; LYMPHOCYTES;
Keywords:
malaria; prime-boost; vaccine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Schneider, J Oxxon Pharmaccines Ltd, Oxford BioBusiness Ctr, Littlemore Pk, Oxford OX4 4SS, England Oxxon Pharmaccines Ltd Littlemore Pk Oxford England OX4 4SS
Citazione:
J. Schneider et al., "A prime-boost immunisation regimen using DNA followed by recombinant modified vaccinia virus Ankara induces strong cellular immune responses against the Plasmodium falciparum TRAP antigen in chimpanzees", VACCINE, 19(32), 2001, pp. 4595-4602

Abstract

Two chimpanzees were vaccinated intramuscularly against malaria using plasmid DNA expressing the pre-erythrocytic antigens thrombospondin related adhesion protein (PfTRAP) and liver stage specific antigen-1 (PfLSA-1) of Plasmodium falciparum together with GM-CSF protein. A recombinant modified vaccinia virus Ankara (MVA) expressing PfTRAP was injected intramuscularly 6 weeks later to boost the immune response. This sequence of antigen delivery induced a specific and long-lasting T cell and antibody response to PfTRAP as detected by ELISPOT assay and ELISA. Antibody responses were detected after four DNA injections, and were boosted by injection of recombinant MVA expressing PfTRAP. Interferon-gamma secreting antigen-specific T cells were detected in both animals, but only after boosting with recombinant MVA. By screening a panel of PfTRAP-derived peptides, an epitope was identified thatwas recognized by cytotoxic T lymphocytes in one of the chimpanzees studied. T cells specific for this epitope were present in PBMCs and liver-infiltrating lymphocytes at a frequency of between 1 in 200 and 1 in 500. The high immunogenicity of this prime-boost regimen in chimpanzees supports further assessment of this delivery strategy for the induction of protection against P. falciparum malaria in humans. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 06:27:31