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Titolo:
Human transferrin receptor gene as a marker gene for MR imaging
Autore:
Moore, A; Josephson, L; Bhorade, RM; Basilion, JP; Weissleder, R;
Indirizzi:
Massachusetts Gen Hosp, Dept Radiol, Ctr Mol Imaging Res, Charlestown, MA 02129 USA Massachusetts Gen Hosp Charlestown MA USA 02129 Charlestown, MA 02129 USA Harvard Univ, Sch Med, Charlestown, MA 02129 USA Harvard Univ CharlestownMA USA 02129 Sch Med, Charlestown, MA 02129 USA
Titolo Testata:
RADIOLOGY
fascicolo: 1, volume: 221, anno: 2001,
pagine: 244 - 250
SICI:
0033-8419(200110)221:1<244:HTRGAA>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
VIRUS THYMIDINE KINASE; SODIUM/IODIDE SYMPORTER GENE; POSITRON-EMISSION-TOMOGRAPHY; IN-VIVO; CONTRAST AGENT; ASIALOGLYCOPROTEIN RECEPTORS; ADENOVIRAL VECTOR; THERAPY MODEL; EXPRESSION; REPORTER;
Keywords:
experimental study; neoplasms, experimental studies; neoplasms, radionuclide studies; genes and genetics;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Moore, A Massachusetts Gen Hosp, Dept Radiol, Ctr Mol Imaging Res, Bldg 149,13th St,Rm 5419, Charlestown, MA 02129 USA Massachusetts Gen Hosp Bldg 149,13th St,Rm 5419 Charlestown MA USA 02129
Citazione:
A. Moore et al., "Human transferrin receptor gene as a marker gene for MR imaging", RADIOLOGY, 221(1), 2001, pp. 244-250

Abstract

PURPOSE: To quantitate and characterize the expression of an engineered human transferrin receptor (ETR) as a marker gene by using magnetic resonance(MR) imaging. MATERIALS AND METHODS: Rat gliosarcoma 9L cells stably expressing ETR (ETR) were used, with nontransfected (ETR-) cells serving as controls. A conjugate of transferrin and monocrystalline iron oxide (Tf-MION) nanoparticles was synthesized to probe for the activity of ETR. Accumulation of Tf-MION was examined by using cell internalization in culture and MR (n = 6) and nuclear (n = 4) imaging in a mouse model with ETR+ and ETR- tumors implanted in the opposite flanks. Autoradiographic and histopathologic results were correlated with MR findings. RESULTS: Tf-MION was internalized by ETR+ cells at 37 degreesC but not at 4 degreesC. Rhodamine-labeled Tf-MION and fluorescein-labeled antibody to ETR colocalized in small vesicle-like structures in the cytoplasm. Both findings were consistent with accumulation by the receptor-mediated endocytosismechanism of ETR. Compared with ETR- tumors, ETR+ tumors accumulated more Tf-MION and had higher signal intensity on T1-weighted MR images and lower signal intensity on T2-weighted images. Autoradiographic findings showed a spatial correlation between MR signal intensity and TF-MION accumulation. CONCLUSION: ETR+ tumors internalize the MR imaging probe through the action of transferrin receptor in amounts that can be detected with MR imaging.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 14:43:38