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Titolo:
Genetic engineering within the adult brain: Implications for molecular approaches to behavioral neuroscience
Autore:
Lowenstein, PR; Castro, MG;
Indirizzi:
Univ Manchester, Sch Med, Mol Med & Gene Therapy Unit, Manchester M13 9PT,Lancs, England Univ Manchester Manchester Lancs England M13 9PT r M13 9PT,Lancs, England
Titolo Testata:
PHYSIOLOGY & BEHAVIOR
fascicolo: 5, volume: 73, anno: 2001,
pagine: 833 - 839
SICI:
0031-9384(200108)73:5<833:GEWTAB>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; LONG-TERM; ADENOVIRAL VECTORS; VII MICE; BETA-GLUCURONIDASE; LENTIVIRAL VECTORS; PARKINSONS-DISEASE; VIRUS VECTORS; HIGH-CAPACITY; IN-VIVO;
Keywords:
viral vectors; adenoviruses; transient transgenesis; regulated expression; gene therapy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Social & Behavioral Sciences
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Lowenstein, PR Cedars Sinai Med Ctr, Gene Therapeut Inst, Suite R-5092,8700 Beverly Blvd,Los Angeles, CA 90048 USA Cedars Sinai Med Ctr Suite R-5092,8700 Beverly Blvd Los Angeles CA USA 90048
Citazione:
P.R. Lowenstein e M.G. Castro, "Genetic engineering within the adult brain: Implications for molecular approaches to behavioral neuroscience", PHYSL BEHAV, 73(5), 2001, pp. 833-839

Abstract

Currently, the most popular technology used to modify the molecular makeupof the nervous system is through germline modifications of early embryos. This allows to construct gene 'knock-ins' (gene overexpression) or 'knock-outs' (gene deletions). This technology leads to gene additions or deletionsfrom the earliest developmental stages. This can potentially lead to compensatory genetic changes. The technology to achieve inducible and cell-type-specific changes in gene expression in transgenic animals has been established. However, it is not yet possible, to reliably turn a particular gene 'on' or 'off' exclusively in adult animals. Alternatively, the use of gene transfer technology in fully mature animals could overcome many of these shortcomings. Gene therapy is the use of nucleic acids as drugs, and uses gene transfer technology to genetically engineer adult animals. Viral and nonviral vectors have been modified to serve as vectors for nucleic acid sequences of interest. Thus, over the last two decades, methods have been developedto deliver particular nucleic acids directly to target tissues. Further technological advances allow delivery of transgenes or antisense mRNAs directly to predetermined cell types, as well as their delivery under the controlof inducible promoter elements. Combined transgenic (i.e., germline modifications) and viral vector technology will also be very powerful in allowingthe genetic modification of selected neuronal populations in adult animals. In this review, we discuss the potential of gene delivery to the brain toanalyze the effect of genetic engineering of particular neuronal groups onbehavior, as well as recent developments and applications of newly engineered vector systems to allow transgenesis within nervous structures of adultanimals. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 18:11:20