Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Role of TGF-beta 1, extracellular matrix, and matrix metalloproteinase in the healing process of the pancreas after induction of acute necrotizing pancreatitis using arginine in rats
Autore:
Kihara, Y; Tashiro, M; Nakamura, H; Yamaguchi, T; Yoshikawa, H; Otsuki, M;
Indirizzi:
Univ Occupat & Environm Hlth, Sch Med, Dept Internal Med 3, Yahatanishi Ku, Kitakyushu, Fukuoka 8078555, Japan Univ Occupat & Environm Hlth Kitakyushu Fukuoka Japan 8078555 8555, Japan
Titolo Testata:
PANCREAS
fascicolo: 3, volume: 23, anno: 2001,
pagine: 288 - 295
SICI:
0885-3177(200110)23:3<288:ROT1EM>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR-BETA; CERULEIN-INDUCED PANCREATITIS; FIBRONECTIN MESSENGER-RNAS; CLOSED DUODENAL LOOP; STELLATE CELLS; TRANSFORMING GROWTH-FACTOR-BETA-1; EXPRESSION; COLLAGEN; REGENERATION; MOUSE;
Keywords:
acute necrotizing pancreatitis; regeneration; TGF-beta 1; extracellular matrix; matrix metalloproteinases;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Otsuki, M Univ Occupat & Environm Hlth, Sch Med, Dept Internal Med 3, Yahatanishi Ku, 1-1 Iseigaoka, Kitakyushu, Fukuoka 8078555, Japan Univ Occupat & Environm Hlth 1-1 Iseigaoka Kitakyushu Fukuoka Japan 8078555
Citazione:
Y. Kihara et al., "Role of TGF-beta 1, extracellular matrix, and matrix metalloproteinase in the healing process of the pancreas after induction of acute necrotizing pancreatitis using arginine in rats", PANCREAS, 23(3), 2001, pp. 288-295

Abstract

Introduction: Pancreatic tissues are almost completely restored to normal after an attack of acute pancreatitis, once the cause of the disease is removed. Transforming growth factor (TGF)-beta and extracellular matrix (ECM) are known to play an important role in the process of wound healing in pathologic diseases. Tissue repair is a process regulated by a balance between synthesis and degradation of ECM. Aims: To elucidate the role of TGF-beta, ECM, and matrix metalloproteinase(MMP) in the process of regeneration occurring after acute necrotizing pancreatitis. Methodology: Acute necrotizing pancreatitis was induced by intraperitonealinjection of 500 mg/100 g body weight of L-arginine in male Wistar rats. Expression of TGF-beta1 and ECM messenger RNA (mRNA) was determined by Northern blot analysis, and that of MMP-1 and MMP-2 mRNA was examined by the reverse transcription polymerase chain reaction (RT-PCR). Immunoreactivity forECM components, TGF-beta1, and MMP-2 in the pancreas was assessed by usinga monoclonal antibody. Results: TGF-beta1 mRNA expression reached a peak value on day 2.5, with adecrease on day 3, and reached the control level on day 7. Procollagen types III and IV and fibronectin mRNA reached a peak value on day 2.5, Whereasthe expression level of procollagen type I mRNA was maximal on day 3, and gradually decreased to control levels by day 7. MMP-2 mRNA was significantly elevated on day 3, and peaked on day 5, Whereas MMP-1 mRNA levels did notchange throughout the observation period. Immunoreactivity for MMP-2 was observed around disrupted acinar cells and interstitial spaces on day 3, andmaximally on day 7. Immunoreactivity for fibronectin was detected around disrupted acinar cells and interstitial spaces. On day 7, it was less than on day 5 around disrupted acinar cells and interstitial spaces, whereas in the regenerated acinar cells, it was undetected. Conclusion: Our results show that TGF-beta1 mRNA expression peaked earlierthan that of ECM mRNA. Furthermore, increased level of the MMP-2 transcript was followed by disappearance of fibronectin. Our findings suggest that TGF-beta1 plays an important role in ECM production in the early phase of acute pancreatitis, and that MMP-2 is involved in the subsequent healing process.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 20:02:26