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Titolo:
In vivo complex formation of PU.1 with HDAC1 associated with PU.1-mediatedtranscriptional repression
Autore:
Kihara-Negishi, F; Yamamoto, H; Suzuki, M; Yamada, T; Sakurai, T; Tamura, T; Oikawa, T;
Indirizzi:
Sasaki Inst, Dept Cell Genet, Chiyoda Ku, Tokyo 1010062, Japan Sasaki Inst Tokyo Japan 1010062 Genet, Chiyoda Ku, Tokyo 1010062, Japan Chiba Univ, Fac Sci, Dept Biol, Inage Ku, Chiba 2638522, Japan Chiba UnivChiba Japan 2638522 Dept Biol, Inage Ku, Chiba 2638522, Japan
Titolo Testata:
ONCOGENE
fascicolo: 42, volume: 20, anno: 2001,
pagine: 6039 - 6047
SICI:
0950-9232(20010920)20:42<6039:IVCFOP>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
MURINE ERYTHROLEUKEMIA-CELLS; THYROID-HORMONE RECEPTOR; HISTONE DEACETYLASE; ACTIVATION DOMAIN; ETS FAMILY; BINDING PROTEIN; EXPRESSION; P300; P53; DIFFERENTIATION;
Keywords:
PU.1; HDAC1; transcriptional repression;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Oikawa, T Sasaki Inst, Dept Cell Genet, Chiyoda Ku, 2-2 Kanda Surugadai, Tokyo 1010062, Japan Sasaki Inst 2-2 Kanda Surugadai Tokyo Japan 1010062 0062, Japan
Citazione:
F. Kihara-Negishi et al., "In vivo complex formation of PU.1 with HDAC1 associated with PU.1-mediatedtranscriptional repression", ONCOGENE, 20(42), 2001, pp. 6039-6047

Abstract

We previously reported that overexpression of PU.1, a member of the Ets family of transcription factors, induces differentiation inhibition and apoptosis associated with c-Myc down-regulation in murine erythroleukemia (MEL) cells. To understand the molecular mechanism by which c-Myc is down-regulated due to overexpression of PU.1, we performed luciferase reporter assays using the mouse c-myc promoter. PU.1 repressed the activities of not only the c-myc promoter but also several other promoters. Experiments with deletion mutants of PU.1 revealed that the C-terminal region spanning amino acids (aa) 123-272 including the PEST and ETS domains but not the activation domain was sufficient for this transcriptional repression. It was unlikely thatthe repression was due to sequestration of a limited amount of CBP/p300 nor pCAF, because overexpression of these co-activators did not relieve PU.1-mediated transcriptional repression. Instead, it was found that the C-terminal aa 101-272 of PU.1 formed a complex with mSin3A and HDAC1 in vivo, which was speculated to be associated with the repression. The C-terminal region of PU.1 also formed a complex with the basic transcription factor TBP in vitro and in vivo. Our results suggest that overexpression of PU.1 induces transcriptional repression in several gene promoters including the c-myc promoter which may be mediated by its complex formation with HDACs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 20:41:24