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Titolo:
Synthesis of selenium-derivatized nucleosides and oligonucleotides for X-ray crystallography
Autore:
Carrasco, N; Ginsburg, D; Du, Q; Huang, Z;
Indirizzi:
CUNY Brooklyn Coll, Dept Chem, Brooklyn, NY 11210 USA CUNY Brooklyn Coll Brooklyn NY USA 11210 ept Chem, Brooklyn, NY 11210 USA CUNY, Grad Sch, Program Biochem & Chem, Brooklyn, NY 11210 USA CUNY Brooklyn NY USA 11210 Program Biochem & Chem, Brooklyn, NY 11210 USA
Titolo Testata:
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
fascicolo: 9, volume: 20, anno: 2001,
pagine: 1723 - 1734
SICI:
1525-7770(2001)20:9<1723:SOSNAO>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
MULTIWAVELENGTH ANOMALOUS DIFFRACTION; CRYSTAL-STRUCTURE; RNA; RIBOZYME; ANALOGS; SITE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Huang, Z CUNY Brooklyn Coll, Dept Chem, 2900 Bedford Ave, Brooklyn, NY 11210 USA CUNY Brooklyn Coll 2900 Bedford Ave Brooklyn NY USA 11210 210 USA
Citazione:
N. Carrasco et al., "Synthesis of selenium-derivatized nucleosides and oligonucleotides for X-ray crystallography", NUCLEOS NUC, 20(9), 2001, pp. 1723-1734

Abstract

We report here the synthesis of nucleoside and oligonucleotide analogs containing selenium, which serves as an anomalous scattering center to enable MAD phase determination in nucleotide X-ray crystallography. We have developed a phase transfer approach to introduce the selenium functionality in A C G, T. and U nucleosides at 5 ' -positions. In the incorporation of the selenium functionality, the leaving groups (bromide, mesyl. and tosyl) were readily displaced by sodium selenide, sodium diselenide, and sodium methyl selenide with yields higher than 90%. Selenium-derivatized oligonucleotides have been synthesized via phosphoramidite chemistry.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 18:22:10