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Titolo:
Identification of tyrosine hydroxylase gene expression in rat spleen
Autore:
Kubovcakova, L; Micutkova, L; Sabban, EL; Krizanova, O; Kvetnansky, R;
Indirizzi:
Slovak Acad Sci, Inst Expt Endocrinol, SK-83306 Bratislava, Slovakia Slovak Acad Sci Bratislava Slovakia SK-83306 -83306 Bratislava, Slovakia New York Med Coll, Dept Biochem & Mol Biol, Valhalla, NY 10595 USA New York Med Coll Valhalla NY USA 10595 Mol Biol, Valhalla, NY 10595 USA
Titolo Testata:
NEUROSCIENCE LETTERS
fascicolo: 2-3, volume: 310, anno: 2001,
pagine: 157 - 160
SICI:
0304-3940(20010914)310:2-3<157:IOTHGE>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
REPEATED IMMOBILIZATION STRESS; MESSENGER-RNA LEVELS; ORNITHINE DECARBOXYLASE; ENZYME-ACTIVITY; CATECHOLAMINES; ADAPTATION; RHYTHMS; YOUNG; NERVE; ASSAY;
Keywords:
tyrosine hydroxylase mRNA; immobilization stress; spleen;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Krizanova, O Slovak Acad Sci, Inst Expt Endocrinol, Vlarska 3, SK-83306 Bratislava, Slovakia Slovak Acad Sci Vlarska 3 Bratislava Slovakia SK-83306 vakia
Citazione:
L. Kubovcakova et al., "Identification of tyrosine hydroxylase gene expression in rat spleen", NEUROSCI L, 310(2-3), 2001, pp. 157-160

Abstract

This study was aimed to identify tyrosine hydroxylase (TH) gene expressionin the rat spleen under basal and stress conditions. Using the reverse transcription polymerase chain,reaction we did not detect TH mRNA in rat spleen either in control, or immobilized animals. Semi-nested PCR revealed a clear signal, demonstrating that TH mRNA is formed in the spleen, although in low abundance. We also detected both, TH immunoreactive protein and TH activity in the rat spleen that were in higher abundance than expected from themRNA levels. This study identifies, for the first time, TH gene expressionin rat spleen. Since TH protein and activity are present in the spleen in much higher abundance compared to corresponding mRNA, the majority of TH protein is most probably supplied by the sympathetic innervation of spleen. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 02:13:51