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Titolo:
Quantal size is dependent on stimulation frequency and calcium entry in calf chromaffin cells
Autore:
Elhamdani, A; Palfrey, HC; Artalejo, CR;
Indirizzi:
Wayne State Univ, Sch Med, Dept Pharmacol, Detroit, MI 48201 USA Wayne State Univ Detroit MI USA 48201 pt Pharmacol, Detroit, MI 48201 USA Univ Chicago, Dept Neurobiol Pharmacol & Physiol, Chicago, IL 60637 USA Univ Chicago Chicago IL USA 60637 rmacol & Physiol, Chicago, IL 60637 USA
Titolo Testata:
NEURON
fascicolo: 5, volume: 31, anno: 2001,
pagine: 819 - 830
SICI:
0896-6273(20010913)31:5<819:QSIDOS>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADRENAL-MEDULLARY CELLS; SYNAPTIC VESICLE MEMBRANE; RAPID ENDOCYTOSIS; TRANSMITTER RELEASE; FUSION PORE; ENDOCRINE-CELLS; EXOCYTOSIS; SECRETION; CATECHOLAMINES; KINETICS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Artalejo, CR Wayne State Univ, Sch Med, Dept Pharmacol, Detroit, MI 48201 USA Wayne State Univ Detroit MI USA 48201 Detroit, MI 48201 USA
Citazione:
A. Elhamdani et al., "Quantal size is dependent on stimulation frequency and calcium entry in calf chromaffin cells", NEURON, 31(5), 2001, pp. 819-830

Abstract

To what extent the quantal hypothesis of transmitter release applies to dense-core vesicle (DCV) secretion is unknown. We determined the characteristics of individual secretory events in calf chromaffin cells using catecholamine amperometry combined with different patterns of stimulation. Raising the frequency of action potential trains from 0.25-10 Hz in 2 mM [Ca2+](o) or [Ca2+](o) from 0.25-7 mM at 7 Hz elevated the amount released per event (quantal size). With increased stimulation, quantal size rose continuously, not abruptly, suggesting that release efficiency from a single population of DCVs rather than recruitment of different-sized vesicles contributed to the effect. These results suggest that catecholamine secretion does not conform to the quantal model. Inhibition of rapid endocytosis damped secretion in successive episodes, implying an essential role for this process in the recycling of vesicles needed for continuous secretion.

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Documento generato il 26/01/21 alle ore 04:29:38