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Titolo:
Endocytosis in rat cultured astrocytes is inhibited by unconjugated bilirubin
Autore:
Silva, RFM; Mata, LM; Gulbenkian, S; Brites, D;
Indirizzi:
Univ Lisbon, Ctr Patogenese Mol, Fac Farm, P-1600083 Lisbon, Portugal UnivLisbon Lisbon Portugal P-1600083 c Farm, P-1600083 Lisbon, Portugal Univ Lisbon, Fac Ciencias, Dept Zool, P-1600214 Lisbon, Portugal Univ Lisbon Lisbon Portugal P-1600214 t Zool, P-1600214 Lisbon, Portugal Gulbenkian Inst Sci, P-2780 Oeiras, Portugal Gulbenkian Inst Sci Oeiras Portugal P-2780 Sci, P-2780 Oeiras, Portugal
Titolo Testata:
NEUROCHEMICAL RESEARCH
fascicolo: 7, volume: 26, anno: 2001,
pagine: 793 - 800
SICI:
0364-3190(200107)26:7<793:EIRCAI>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
3-(4,5-DIMETHYLTHIAZOL-2-YL)-2,5-DIPHENYLTETRAZOLIUM BROMIDE MTT; FIBRILLARY ACIDIC PROTEIN; CELL-LINE N-115; INTERMEDIATE FILAMENTS; FORMAZAN EXOCYTOSIS; AMYLOID PEPTIDES; TERM INFANTS; TOXICITY; BRAIN; REDUCTION;
Keywords:
cationized ferritin; cell endocytosis; cytotoxicity; cytoskeletal microtubules and filaments; unconjugated bilirubin; rat cortical astrocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Brites, D Univ Lisbon, Ctr Patogenese Mol, Fac Farm, Av Forcas Armadas, P-1600083 Lisbon, Portugal Univ Lisbon Av Forcas Armadas Lisbon Portugal P-1600083 ortugal
Citazione:
R.F.M. Silva et al., "Endocytosis in rat cultured astrocytes is inhibited by unconjugated bilirubin", NEUROCHEM R, 26(7), 2001, pp. 793-800

Abstract

Excessive hyperbilirubinemia can cause irreversible neurological damage inthe neonatal period. However, the complete understanding of the pathogenesis of unconjugated bilirubin (UCB) encephalopathy remains a matter of debate. This study investigates whether UCB inhibits the endocytosis of cationized ferritin (CF) by cultured rat astrocytes. The relationship between endocytosis and MTT reduction, as well as changes on tubulin and glial fibrillary acidic protein (GFAP) assembly, were also evaluated. Inhibition of endocytosis was complete in the presence of 171 muM UCB, while a marked decrease of CF labeling was noticed for 86 PM UCB. In addition, MTT reduction was inhibited by 60 to 76% as UCB concentrations changed from 17 to 171 muM, while alterations on both GFAP and microtubule morphology were only achieved bycell exposure to 171 muM UCB. These findings indicate that inhibition of CF endocytosis in rat cortical astrocytes by UCB is a concentration-dependent process that appears to be primarily related to a direct effect on the cell membrane and not to any alteration of cytoskeletal microtubules and intermediate filaments.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/21 alle ore 14:11:01