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Titolo:
Association between genetic polymorphisms and biomarkers in styrene-exposed workers
Autore:
Vodicka, P; Soucek, P; Tates, AD; Dusinska, M; Sarmanova, J; Zamecnikova, M; Vodickova, L; Koskinen, M; de Zwart, FA; Natarajan, AT; Hemminki, K;
Indirizzi:
Acad Sci Czech Republ, Inst Expt Med, Prague 14220 4, Czech Republic Acad Sci Czech Republ Prague Czech Republic 14220 4 20 4, Czech Republic Natl Inst Publ Hlth, Prague 10042 10, Czech Republic Natl Inst Publ Hlth Prague Czech Republic 10042 10 42 10, Czech Republic Leiden Univ, Med Ctr, MGC, Dept Radiat Genet & Chem Mutagenesis, NL-2333 RA Leiden, Netherlands Leiden Univ Leiden Netherlands NL-2333 RA NL-2333 RA Leiden, Netherlands Inst Prevent & Clin Med, Bratislava 83301, Slovakia Inst Prevent & Clin Med Bratislava Slovakia 83301 islava 83301, Slovakia Natl Inst Publ Hlth, Bratislava, Slovakia Natl Inst Publ Hlth BratislavaSlovakia Publ Hlth, Bratislava, Slovakia Karolinska Inst, Novum, Dept Biosci, S-14157 Huddinge, Sweden Karolinska Inst Huddinge Sweden S-14157 Biosci, S-14157 Huddinge, Sweden
Titolo Testata:
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
fascicolo: 1-2, volume: 482, anno: 2001,
pagine: 89 - 103
SICI:
1386-1964(20011001)482:1-2<89:ABGPAB>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
HPRT MUTANT FREQUENCY; SISTER-CHROMATID EXCHANGES; SINGLE-STRAND BREAKS; LUNG-CANCER; LAMINATION WORKERS; T-LYMPHOCYTES; DNA-ADDUCTS; CYTOCHROME-P450IA1 GENE; REINFORCED-PLASTICS; CIGARETTE-SMOKING;
Keywords:
occupational exposure to styrene; genetic polymorphisms; DNA damage; HPRT mutant frequency; chromosomal aberrations;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Vodicka, P Acad Sci Czech Republ, Inst Expt Med, Videnska 1083, Prague 14220 4, CzechRepublic Acad Sci Czech Republ Videnska 1083 Prague Czech Republic 14220 4
Citazione:
P. Vodicka et al., "Association between genetic polymorphisms and biomarkers in styrene-exposed workers", MUT RES-F M, 482(1-2), 2001, pp. 89-103

Abstract

A comprehensive approach to evaluate genotoxic effects induced by styrene exposure was employed in 44 hand-lamination workers in comparison with 18 unexposed controls. The acquired data on single-strand breaks in DNA (SSBs),frequency of chromosomal aberrations and HPRT mutant frequency in peripheral blood lymphocytes were compared to the results on genotyping of some of the xenobiotic-metabolising enzymes (CYP1A1, CYP2E1, epoxide hydrolase and GSTM1, GSTP1 and GSTT1), Multifactorial regression analysis indicated that SSB in DNA were significantly associated with styrene exposure and with heterozygosity in CYP2E1 (5 ' -flanking region and intron 6; r(2) = 0.614). The frequency of chromosomal abet-rations (CA), as analysed by linear multiple regression analysis, significantly correlated with years of employment (P= 0.004) and with combinations of epoxide hydrolase (EPHX) genotypes (exon3, Tyr/His and exon 4, His/Arg), where individuals with low and medium activity EPHX genotypes exhibited higher frequencies of CA than those with high activity genotypes (P = 0.044, r(2) = 0.563). Moderately higher HPRT mutant frequencies were detected in styrene-exposed individuals (20.2 +/- 25.8 x 10(-6)) as compared to controls (13.3 +/- 6.3 x 10(-6)), but this difference was not significant. ANOVA (in the whole set of data) revealed that mutant frequencies at the HPRT gene were significantly associated with years of employment (F = 6.9 P = 0.0001), styrene in blood (F = 10.1, P = 0.0001),and heterozygosity in CYP2E1 (intron 6; F = 13.5, P = 0.0008) and GSTP1 (exon 5; F = 3.6, P = 0.038). In conclusion, our present data suggest that analysed biomarkers of DNA damage may be modulated by polymorphic CYP2E1, EPHX and GSTP1. In our study, styrene-specific DNA and haemoglobin adducts are under investigation. Completing these data with the results of genotyping of metabolising enzymes mayprovide a useful toot for individual genotoxic risk assessment. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 03:18:00