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Titolo:
Membrane raft-dependent regulation of phospholipase C gamma-1 activation in T lymphocytes
Autore:
Veri, MC; DeBell, KE; Seminario, MC; DiBaldassarre, A; Reischl, I; Rawat, R; Graham, L; Noviello, C; Rellahan, BL; Miscia, S; Wange, RL; Bonvini, E;
Indirizzi:
Ctr Biol Evaluat & Res, Immunobiol Lab, Div Monoclonal Antibodies, LIB,OTRR, Bethesda, MD 20892 USA Ctr Biol Evaluat & Res Bethesda MD USA 20892 OTRR, Bethesda, MD 20892 USA NIAMSD, Arthrit & Rheumatism Branch, NIH, Bethesda, MD 20892 USA NIAMSD Bethesda MD USA 20892 eumatism Branch, NIH, Bethesda, MD 20892 USA NIA, Gerontol Res Ctr, Biol Chem Lab, NIH, Baltimore, MD 21224 USA NIA Baltimore MD USA 21224 r, Biol Chem Lab, NIH, Baltimore, MD 21224 USA Univ G DAnnunzio, Ist Morfol Umana Normale, I-66100 Chieti, Italy Univ G DAnnunzio Chieti Italy I-66100 ana Normale, I-66100 Chieti, Italy
Titolo Testata:
MOLECULAR AND CELLULAR BIOLOGY
fascicolo: 20, volume: 21, anno: 2001,
pagine: 6939 - 6950
SICI:
0270-7306(200110)21:20<6939:MRROPC>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL ANTIGEN RECEPTOR; PROTEIN-TYROSINE KINASE; TEC FAMILY KINASES; SIGNAL-TRANSDUCTION; PLASMA-MEMBRANE; GENETIC-EVIDENCE; PHOSPHORYLATION; ZAP-70; LAT; LOCALIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Bonvini, E Ctr Biol Evaluat & Res, Immunobiol Lab, Div Monoclonal Antibodies, LIB,OTRR, HFM-564,Bldg 29B,Rm 3NN10,29 Lincoln Dr,MSC-4555, Bethesda, MD 20892 USA Ctr Biol Evaluat & Res HFM-564,Bldg 29B,Rm 3NN10,29 Lincoln Dr,MSC-4555 Bethesda MD USA 20892
Citazione:
M.C. Veri et al., "Membrane raft-dependent regulation of phospholipase C gamma-1 activation in T lymphocytes", MOL CELL B, 21(20), 2001, pp. 6939-6950

Abstract

Numerous signaling molecules associate with lipid rafts, either constitutively or after engagement of surface receptors. One such molecule, phospholipase C gamma -1 (PLC gamma1), translocates from the cytosol to lipid rafts during T-cell receptor (TCR) signaling. To investigate the role played by lipid rafts in the activation of this molecule in T cells, an influenza virus hemagglutinin A (HA)-tagged PLC gamma1 was ectopically expressed in Jurkat T cells and targeted to these microdomains by the addition of a dual-acylation signal. Raft-targeted PLC gamma1 was constitutively tyrosine phosphorylated and induced constitutive NF-AT-dependent transcription and interleukin-2 secretion in Jurkat cells. Tyrosine phosphorylation of raft-targeted PLC gamma1 did not require Zap-70 or the interaction with the adapters Lat and Slp-76, molecules that are necessary for TCR signaling. In contrast, theSrc family kinase Lek was required. Coexpression in HEK 293T cells of PLC gamma1-HA with Lek or the Tec family kinase Rlk resulted in preferential phosphorylation of raft-targeted PLC gamma1 over wild-type PLC gamma1. These data show that localization of PLC gamma1 in lipid rafts is sufficient for its activation and demonstrate a role for lipid rafts as microdomains that dynamically segregate and integrate PLC gamma1 with other signaling components.

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Documento generato il 30/11/20 alle ore 16:38:41