Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Gene transfer into retinal ganglion cells by in vivo electroporation: a new approach
Autore:
Dezawa, M; Takano, M; Negishi, H; Mo, XF; Oshitari, T; Sawada, H;
Indirizzi:
Yokohama City Univ, Sch Med, Dept Anat, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan Yokohama City Univ Yokohama Kanagawa Japan 2360004 anagawa 2360004, Japan Yokohama City Univ, Sch Med, Dept Ophthalmol, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan Yokohama City Univ Yokohama Kanagawa Japan 2360004 anagawa 2360004, Japan Chiba Univ, Sch Med, Dept Ophthalmol, Chuo Ku, Chiba 2608670, Japan Chiba Univ Chiba Japan 2608670 Ophthalmol, Chuo Ku, Chiba 2608670, Japan
Titolo Testata:
MICRON
fascicolo: 1, volume: 33, anno: 2002,
pagine: 1 - 6
SICI:
0968-4328(2002)33:1<1:GTIRGC>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VIVO; DNA INJECTION; RAT-LIVER; THERAPY; PARAMETERS; LIPOSOMES; PROTEIN; TISSUES; PULSE;
Keywords:
eye; retina; vision; electric pulse; green fluorescent protein; in vivo gene therapy;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Dezawa, M Yokohama City Univ, Sch Med, Dept Anat, Kanazawa Ku, 3-9 Fukuura, Yokohama, Kanagawa 2360004, Japan Yokohama City Univ 3-9 Fukuura YokohamaKanagawa Japan 2360004 n
Citazione:
M. Dezawa et al., "Gene transfer into retinal ganglion cells by in vivo electroporation: a new approach", MICRON, 33(1), 2002, pp. 1-6

Abstract

We developed a new in vivo electroporation. method to deliver genes into retinal ganglion cells (RGCs). Efficiency and degree of tissue damage were evaluated using green fluorescent protein (GFP) gene and TUNEL. Soon after the intravitreous injection of the GFP gene, electroporation (five electric pulses of 99 ms duration each and 12 V/cm. delivered twice 5 min apart) wascarried out on the adult rat eyeball with the aid of tweezer-type disc electrodes attached to corneal (cathode) and scleral (anode) surfaces. GFP expression, exhibiting a maximum on day 7, was detectable for up to 21 days. DiI retrograde labeling of RGCs showed that 41.5% of the total ganglion cells in the electroinjected area were GFP-positive. Therefore, this new methodmay be a useful tool for the delivery of genes into RGCs. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 00:20:16