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Titolo:
Comparative study on sodium transport and Na+,K+-ATPase activity in Caco-2and rat jejunal epithelial cells: Effects of dopamine
Autore:
Vieira-Coelho, MA; Soares-da-Silva, P;
Indirizzi:
Fac Med Porto, Inst Pharmacol & Therapeut, P-4200 Oporto, Portugal Fac MedPorto Oporto Portugal P-4200 Therapeut, P-4200 Oporto, Portugal
Titolo Testata:
LIFE SCIENCES
fascicolo: 17, volume: 69, anno: 2001,
pagine: 1969 - 1981
SICI:
0024-3205(20010914)69:17<1969:CSOSTA>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
NA+-K+-ATPASE; LINE CACO-2; SMALL-INTESTINE; RECEPTORS; EXPRESSION; KIDNEY; COLON; HYPERTENSION; BINDING; MUCOSA;
Keywords:
dopamine; Caco-2 cells; intestine; rat; sodium;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Soares-da-Silva, P Fac Med Porto, Inst Pharmacol & Therapeut, P-4200 Oporto, Portugal Fac Med Porto Oporto Portugal P-4200 Oporto, Portugal
Citazione:
M.A. Vieira-Coelho e P. Soares-da-Silva, "Comparative study on sodium transport and Na+,K+-ATPase activity in Caco-2and rat jejunal epithelial cells: Effects of dopamine", LIFE SCI, 69(17), 2001, pp. 1969-1981

Abstract

The present study reports on the effects of dopamine on sodium transepithelial transport and Na+,K+-ATPase activity in Caco-2 cells, a human epithelial intestinal cell line which undergoes enterocyte differentiation in culture, and jejunal epithelial cells from 20 day old Wistar rats. Addition of amphotericin B to the mucosal side stimulated I-sc in a concentration dependent manner (Caco-2 cells, EC50=0.9 [0.5, 1.7] muM; rat jejunum, EC50=7.4 [0.8; 70.1] muM). The presence of 1 muM dopamine did not change the effect ofamphotericin B in Caco-2 cells, but produced a significant (P <0.05) decrease in the maximal effect of amphotericin B in the rat jejunum. Dopamine (1muM), added to the serosal side, did not change the I-sc profile in Caco-2cells, but produced a significant increase in the (mu)rat jejunum. This effect was antagonized by SKF 83566 (1 muM), but not S-sulpiride (1 muM), andwas mimicked by SKF 38393 (10 nM), but not by quinerolane (10 nM). Basal Na+, K+-ATPase activity (in nmol Pi mg protein(-1) min(-1)) in Caco-2 cells (49.5 +/-0.2) was similar to that observed in isolated rat jejunal epithelial cells (52.3 +/-3.4). Dopamine (1 muM) significantly (P <0.05) decreased Na+, K+-ATPase activity in rat jejunal epithelial cells, but failed to inhibit Na+,K+-ATPase in Caco-2 cells. This effect of dopamine was antagonized by SKF 83566 (1 M), but not S-sulpiride (1 muM), and was mimicked by SKF 38393 (10 nM), but not by quinerolane (10 muM). The specific binding of [H-3]-Sch 23390 to the rat intestinal mucosa was saturable with an apparent dissociation constant (K-D) of 2.4 (0.4; 4.5) nM and maximum receptor density of 259.8 +/- 32.6 fmol/mg protein. No significant specific binding of [H-3]-Sch 23390 was observed in membranes from Caco-2 cells. In conclusion, the results obtained show that D-1-like receptor mediated effects of dopamine inthe rat jejunum on sodium absorption are absent in Caco-2 cells, most probably because this cell line does not express DI-like dopamine receptors, which ultimately are responsible for the inhibitory effect of the amine upon intestinal Na+,K+-ATPase. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 16:37:17